BBa_K597100 1 BBa_K597100 avi-RFP [AviTagged red fluorescent protein] 2011-09-24T11:00:00Z 2015-05-08T01:12:49Z Avidity, L.L.C., Eleanor Roosevelt Institute, Denver, Colorado 80206, USA. Ref: Cull, M.G. and Schat, P.J. (2000) "Biotinylation of proteins in vivo and in vitro using small peptide tags." | Methods of Enzymology Vol 326, 2000, pages 430???440 Avi-RFP codes for RFP with an appended biotinylation marker. When attached to a promoter, it can be used as a red fluorescent reporter which will be biotinylated by E coii. if cultured with biotin. This reporter can then be used in biotin-streptavidin binding applications. true false _769_ 0 10191 9 Discontinued false Avitag sequence was taken from Cull and Schat, 2000. false Claire Paduano annotation2139527 1 Avitag range2139527 1 688 732 annotation2139526 1 RFP range2139526 1 1 675 annotation2139529 1 start codon range2139529 1 1 3 annotation2139528 1 RFP range2139528 1 733 735 BBa_K597100_sequence 1 atggcctcctccgaggacgtcatcaaggagttcatgcgcttcaaggtgcgcatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtcccctcagttccagtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccaccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagatgaggctgaagctgaaggacggcggccactacgacgccgaggtcaagaccacctacatggccaagaagcccgtgcagctgcccggcgcctacaagaccgacatcaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtacgagcgcgccgagggccgccactccaccggcgccaagcttgcatgcggcctgaacgacatcttcgaggctcagaaaatcgaatggcacgaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z