BBa_K598001
1
BBa_K598001
Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
2011-09-26T11:00:00Z
2015-05-08T01:12:50Z
Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
Released HQ 2013
This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
false
false
_770_
0
8713
9
In stock
true
The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
false
Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
annotation2152887
1
polya
range2152887
1
930
943
annotation2152704
1
eGFP
range2152704
1
87
806
annotation2152703
1
BBa_E0040
range2152703
1
87
806
annotation2152901
1
T7 TE
range2152901
1
910
929
annotation2152892
1
stop
range2152892
1
936
936
annotation2152705
1
BBa_B0012
range2152705
1
903
943
annotation2152701
1
Theophylline responsive riboswitch Theo 1G1
range2152701
1
1
86
annotation2152702
1
Engineered RBS
range2152702
1
75
81
annotation2152882
1
BBa_B0010
range2152882
1
815
894
annotation2152895
1
stem_loop
range2152895
1
826
869
BBa_K598001_sequence
1
tccatgtatgttgatacttaatttaaagattaaacaaaagatgataccagccgaaaggcccttggcagctctcgaggaggtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z