BBa_K598001 1 BBa_K598001 Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator 2011-09-26T11:00:00Z 2015-05-08T01:12:50Z Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform. Released HQ 2013 This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes. false false _770_ 0 8713 9 In stock true The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well. false Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG annotation2152882 1 BBa_B0010 range2152882 1 815 894 annotation2152703 1 BBa_E0040 range2152703 1 87 806 annotation2152705 1 BBa_B0012 range2152705 1 903 943 annotation2152901 1 T7 TE range2152901 1 910 929 annotation2152701 1 Theophylline responsive riboswitch Theo 1G1 range2152701 1 1 86 annotation2152702 1 Engineered RBS range2152702 1 75 81 annotation2152887 1 polya range2152887 1 930 943 annotation2152892 1 stop range2152892 1 936 936 annotation2152895 1 stem_loop range2152895 1 826 869 annotation2152704 1 eGFP range2152704 1 87 806 BBa_K598001_sequence 1 tccatgtatgttgatacttaatttaaagattaaacaaaagatgataccagccgaaaggcccttggcagctctcgaggaggtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z