LOCUS       BBa_K598011             1144 bp    DNA     linear       12-May-2024
DEFINITION  pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native 
            RBS+E0040+B0015
ACCESSION   BBa_K598011
VERSION     BBa_K598011.1  
FEATURES             Location/Qualifiers
     RBS             275..282
                     /label=RBS
     CDS             288..1007
                     /label=eGFP
     promoter        1..130
                     /label=pBAD
     misc_feature    137..287
                     /label=TPP Ribozyme 2.5
     misc_feature    288..1007
                     /label=BBa_E0040
     misc_feature    1016..1144
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagattc tccttcggta catccagctg atgagtccca aataggacga
      181 aatcctcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
      241 aatgccagcg tagggatgga tcctggattc cacgaaggag atataccatg cgtaaaggag
      301 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      361 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      421 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      481 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      541 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      601 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      661 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      721 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      781 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      841 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      901 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      961 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1141 tata
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