LOCUS       BBa_K598016             1095 bp    DNA     linear       23-Dec-2024
DEFINITION  T7-promoter+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native 
            RBS+GFP+T7-terminator
ACCESSION   BBa_K598016
VERSION     BBa_K598016.1  
FEATURES             Location/Qualifiers
     promoter        1..47
                     /label=T7
     CDS             201..1048
                     /label=GFP
     misc_feature    48..200
                     /label=TPP Down-regulate Hammerhead Ribozyme 2.5
     RBS             187..195
                     /label=RBS
     misc_feature    1056..1095
                     /label=T7
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctttc tccttcggta
       61 catccagctg atgagtccca aataggacga aatcctcggg gtgcccttct gcgtgaaggc
      121 tgagaaatac ccgtatcacc tgatctggat aatgccagcg tagggatgga ggatcctgga
      181 ttccacgaag gagatatacc atgggccatc atcatcatca tcatcatcat catcacagca
      241 gcggccatat cgaaggtcgt catatggtga gcaagggcga ggagctgttc accggggtgg
      301 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      361 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      421 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      481 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      541 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      601 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      661 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      721 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      781 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      841 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
      901 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      961 gcatggacga gctgtacaag taaggatccg gctgctaaca aagcccgaaa ggaagctgag
     1021 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1081 ttgaggggtt ttttg
//