LOCUS BBa_K598019 6710 bp DNA linear 04-May-2024 DEFINITION B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE ACCESSION BBa_K598019 VERSION BBa_K598019.1 FEATURES Location/Qualifiers stem_loop 1..129 /label=B0015 RBS 4565..4571 /label=rbs promoter 138..267 /label=pBAD RBS 273..279 /label=rbs CDS 285..1542 /label=vioA RBS 1545..1551 /label=rbs CDS 1559..4556 /label=vioB CDS 4579..5704 /label=vioD stem_loop 5712..5841 /label=B0015 promoter 5849..5978 /label=pBAD misc_feature 5985..6134 /label=TPP ribozyme 2.5 RBS 6122..6129 /label=rbs CDS 6135..6710 /label=vioE ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagaca ttgattattt gcacggcgtc acactttgct atgccatagc 181 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt 241 ttctccatac cgtttttttg ggctagcact agaggaggta ctagatgaaa cattcttccg 301 atatctgcat tgttggtgct ggtatttctg gtttgacgtg cgcaagccat ctgctggaca 361 gcccggcatg ccgtggtctg agcctgcgta tctttgacat gcagcaagaa gccggtggcc 421 gtatccgcag caaaatgctg gatggtaagg caagcattga actgggcgca ggtcgctact 481 cccctcagtt gcacccgcat ttccaaagcg caatgcagca ctatagccaa aagagcgaag 541 tctatccgtt cacccagttg aagttcaaat ctcacgtgca gcaaaagctg aagcgcgcca 601 tgaatgaact gtccccgcgt ctgaaagagc atggtaaaga gagctttttg cagtttgtca 661 gccgttatca aggtcacgat agcgcggttg gtatgatccg ctctatgggt tacgacgcac 721 tgttcctgcc ggatatcagc gcagaaatgg cctacgacat tgtgggtaag cacccggaga 781 tccagagcgt gacggacaac gacgcgaacc aatggtttgc agcggaaacg ggctttgctg 841 gtctgattca gggcatcaag gctaaggtta aggcggcagg tgcgcgtttt agcctgggtt 901 atcgtctgct gagcgtccgt accgacggtg acggctacct gctgcaactg gcaggtgacg 961 acggctggaa actggagcac cgtacccgcc atctgattct ggcgattccg ccgagcgcga 1021 tggcgggttt gaatgttgat tttccagaag cctggtccgg tgcgcgctat ggcagcctgc 1081 cgctgtttaa gggctttctg acgtacggtg agccgtggtg gttggactac aaactggacg 1141 atcaggtgct gattgttgac aacccgctgc gcaaaatcta tttcaaaggc gataagtacc 1201 tgttcttcta taccgatagc gagatggcga attactggcg cggttgtgtc gcggagggcg 1261 aggacggtta cctggagcaa attcgcaccc atttggctag cgcactgggt atcgtccgtg 1321 aacgtatccc gcaaccgctg gcacacgttc acaagtattg ggcgcacggc gttgagtttt 1381 gccgtgattc tgatattgac cacccgagcg cactgtctca tcgcgacagc ggtatcatcg 1441 cgtgctccga tgcgtacacg gagcattgtg gttggatgga gggcggtctg ctgagcgccc 1501 gtgaggcaag ccgtctgctg ttgcagcgta tcgccgcgtg attaaggagg taaaaaaaat 1561 gagcattctg gatttcccgc gtatccactt ccgtggctgg gcccgtgtca atgcgccgac 1621 cgcgaaccgc gatccgcacg gccacatcga tatggccagc aataccgtgg cgatggcggg 1681 tgagccgttc gacctggcac gccatcctac ggagttccac cgtcacctgc gctccctggg 1741 tccgcgcttc ggcttggatg gtcgtgctga cccggaaggc ccgttcagcc tggccgaggg 1801 ctacaacgct gccggtaaca accacttttc gtgggagagc gcaaccgtta gccacgtgca 1861 atgggatggc ggtgaggcgg atcgtggtga cggtctggtc ggtgctcgtt tggcactgtg 1921 gggtcactac aatgattatc tgcgtaccac cttcaatcgt gctcgttggg tcgacagcga 1981 cccgacgcgc cgtgacgctg cacaaatcta tgcgggccaa ttcaccatta gcccggctgg 2041 tgccggtccg ggtacgccgt ggctgtttac ggcagacatt gatgatagcc atggtgcacg 2101 ttggacgcgt ggcggccaca ttgcagagcg tggcggccac ttcttggatg aagagtttgg 2161 tctggcacgc ctgtttcagt tctctgtgcc gaaagatcac ccacattttc tgtttcaccc 2221 gggtccgttt gattccgagg cctggcgtcg tctgcaattg gctctggagg atgacgacgt 2281 tctgggtctg accgtgcaat atgcgttgtt caatatgagc accccgcctc agccgaacag 2341 cccggttttt cacgatatgg tcggtgttgt cggtctgtgg cgtcgtggtg aactggcgag 2401 ctacccggct ggtcgtctgc tgcgtccgcg tcaaccgggt ctgggtgacc tgaccctgcg 2461 cgtcaacggt ggtcgcgttg cgctgaattt ggcgtgtgcc attccgttca gcactcgtgc 2521 cgcgcagcca agcgcaccgg accgcctgac cccggacctg ggtgccaaac tgccgctggg 2581 cgatctgctg ctgcgtgatg aggacggcgc actgttggca cgtgtgccgc aggctctgta 2641 ccaagactat tggacgaatc acggtattgt ggacctgccg ctgctgcgcg aaccgcgtgg 2701 tagcttgacc ctgagcagcg aactggcgga gtggcgtgag caagactggg tcacccaaag 2761 cgacgcgtct aacctgtacc tggaggcacc ggatcgccgt cacggtcgct ttttccctga 2821 gagcatcgcg ctgcgcagct actttcgcgg tgaagcgcgt gcgcgtccgg atatcccgca 2881 tcgtatcgag ggcatgggcc tggtcggcgt cgaatctcgt caggatggcg acgctgcgga 2941 atggcgtctg acgggtctgc gtccgggtcc ggcacgcatt gttctggacg atggtgccga 3001 ggcgatccct ctgcgtgttc tgcctgacga ttgggcgctg gatgacgcga ccgtcgaaga 3061 agtggattac gcctttttgt accgccacgt tatggcgtat tacgagctgg tgtatccatt 3121 catgagcgac aaggtgtttt ccctggctga tcgttgcaaa tgtgaaacgt acgcacgtct 3181 gatgtggcag atgtgtgatc cgcagaaccg caacaagtcc tattacatgc cgagcacccg 3241 cgaactgtcg gcaccgaaag ctcgtttgtt cttgaagtat ctggcccacg tggaaggcca 3301 ggcacgcctg caagcacctc cgccagcggg tccggcacgc attgaatcta aagcccagtt 3361 ggcggcagag ctgcgtaaag ccgtcgacct ggagctgtct gtgatgctgc aatacctgta 3421 cgcggcgtat agcattccga actatgcaca gggccaacaa cgtgttcgtg acggtgcgtg 3481 gaccgccgag cagctgcaac tggcgtgcgg tagcggtgac cgtcgccgtg atggcggtat 3541 tcgtgcagca ctgctggaaa ttgctcatga agaaatgatt cattacctgg tcgttaacaa 3601 cctgctgatg gccctgggcg agccgttcta cgcgggtgtc ccgctgatgg gcgaagcggc 3661 acgtcaggcg tttggcctgg acaccgagtt cgctctggaa ccgtttagcg aaagcacgct 3721 ggcacgtttt gttcgtctgg aatggccgca ctttatccca gcaccgggca aatccatcgc 3781 ggactgctat gccgccattc gtcaggcgtt tttggatctg ccggacttgt ttggtggcga 3841 ggcaggtaag cgtggcggtg aacaccacct gttcctgaat gagctgacca accgtgcgca 3901 tccgggttat caactggaag ttttcgatcg cgactcggcg ctgtttggta ttgcatttgt 3961 gaccgatcag ggcgaaggtg gcgctctgga cagcccgcac tacgaacata gccattttca 4021 acgtctgcgt gaaatgagcg cgcgtatcat ggctcaaagc gcaccgttcg aaccggcgct 4081 gccggcgttg cgtaatccgg ttctggatga gagcccgggt tgccaacgtg tcgcagacgg 4141 tcgtgcgcgt gcgctgatgg cattgtacca aggcgtttat gagctgatgt ttgcgatgat 4201 ggcgcagcac ttcgccgtga aaccgctggg tagcttgcgt cgcagccgcc tgatgaacgc 4261 agcaatcgat ctgatgaccg gtctgttgcg tccgctgagc tgcgcgctga tgaacctgcc 4321 aagcggcatc gccggtcgca cggccggtcc gccgctgccg ggtccggttg acacccgtag 4381 ctatgacgac tacgcgctgg gctgtcgcat gctggcacgc cgttgcgagc gtctgctgga 4441 gcaggcgagc atgctggaac cgggttggct gccggatgcg cagatggagc tgctggattt 4501 ctatcgtcgc caaatgctgg acttggcgtg cggcaaactg agccgcgagg cctaaggatc 4561 tttaaggagg taaaaaaaat gaagattctg gtcattggtg ctggtccagc tggtctggtt 4621 ttcgcatccc aactgaagca ggcacgccct ttgtgggcca ttgacatcgt ggagaagaat 4681 gacgagcaag aagtgctggg ctggggtgtc gtgctgcctg gccgtccggg tcagcacccg 4741 gcgaacccgc tgtcctatct ggatgcaccg gagcgtctga atccgcaatt tctggaggac 4801 ttcaaactgg tgcatcataa tgagccgtcc ttgatgtcca cgggcgtttt gttgtgcggc 4861 gtggagcgtc gcggtctggt tcacgcgctg cgcgataagt gccgcagcca aggcattgct 4921 attcgtttcg aaagcccgtt gctggaacac ggtgagctgc cgctggcgga ctatgatctg 4981 gtggtcctgg ctaatggtgt taatcacaaa accgcgcatt tcaccgaggc tctggtcccg 5041 caggtggact acggccgcaa taagtacatt tggtatggca ctagccagct gttcgatcag 5101 atgaatctgg tttttcgtac ccatggtaaa gatatcttta tcgcgcatgc ctataagtat 5161 agcgatacca tgagcacgtt cattgtcgaa tgtagcgaag agacttacgc acgcgcacgc 5221 ctgggcgaaa tgtccgaaga ggcgagcgca gaatacgttg ctaaggtgtt ccaggccgag 5281 ctgggtggtc acggcctggt gagccagccg ggtctgggtt ggcgtaactt catgacgttg 5341 tctcatgacc gttgtcatga tggtaagttg gttctgctgg gtgacgcgct gcaaagcggt 5401 cactttagca tcggccacgg caccacgatg gccgtggtgg tggcgcagct gctggttaaa 5461 gcgctgtgta ccgaagatgg tgtgcctgcc gcgctgaaac gtttcgaaga gcgtgccctg 5521 ccgctggtgc agttgttccg tggccacgca gacaacagcc gcgtttggtt cgaaaccgtc 5581 gaagagcgca tgcacctgtc ctcggcggaa tttgtgcaaa gcttcgacgc acgccgcaaa 5641 agcctgccgc cgatgccgga agcactggcg cagaatctgc gttatgcttt gcagcgctga 5701 tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5761 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5821 tgggcctttc tgcgtttata tactagagac attgattatt tgcacggcgt cacactttgc 5881 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa 5941 ctctctactg tttctccata ccgttttttt gggctagcac tagattctcc ttcggtacat 6001 ccagctgatg agtcccaaat aggacgaaat cctcggggtg cccttctgcg tgaaggctga 6061 gaaatacccg tatcacctga tctggataat gccagcgtag ggatggatcc tggattccac 6121 gaaggagata taccatggag aaccgtgagc caccactgtt gccagcccgt tggagcagcg 6181 cctatgtctc ttattggagc ccgatgctgc cggatgacca gctgaccagc ggctattgct 6241 ggttcgacta tgaacgtgac atctgtcgta ttgacggcct gttcaatccg tggagcgagc 6301 gtgatactgg ttatcgcctg tggatgtcgg aggttggtaa tgcggccagc ggccgtacct 6361 ggaaacaaaa agtcgcctat ggtcgtgagc gtaccgccct gggtgaacag ctgtgtgagc 6421 gtccgctgga tgatgagact ggcccttttg ccgaattgtt cctgccacgc gatgtcctgc 6481 gccgtctggg tgcccgtcac attggccgtc gcgtggttct gggtcgcgaa gcggacggtt 6541 ggcgttacca gcgcccaggt aaaggtccga gcaccctgta cctggatgcg gcgagcggca 6601 ctccactgcg catggtcacc ggcgatgaag cgtcgcgtgc aagcctgcgt gattttccga 6661 atgtgagcga ggcggagatc ccggacgcgg ttttcgcggc caagcgctaa //