##gff-version 3
##sequence-region BBa_K598019 1 6710
BBa_K598019	.	CDS	6135	6710	.	+	0	ID=annotation2144631;Name=vioE
BBa_K598019	.	CDS	1559	4556	.	+	0	ID=annotation2144599;Name=vioB
BBa_K598019	.	ribosome_entry_site	1545	1551	.	+	0	ID=annotation2144598;Name=rbs
BBa_K598019	.	CDS	285	1542	.	+	0	ID=annotation2144597;Name=vioA
BBa_K598019	.	engineered_region	5985	6134	.	+	0	ID=annotation2144624;Name=TPP ribozyme 2.5
BBa_K598019	.	ribosome_entry_site	273	279	.	+	0	ID=annotation2144596;Name=rbs
BBa_K598019	.	CDS	4579	5704	.	+	0	ID=annotation2144600;Name=vioD
BBa_K598019	.	ribosome_entry_site	6122	6129	.	+	0	ID=annotation2144630;Name=rbs
BBa_K598019	.	stem_loop	1	129	.	+	0	ID=annotation2144593;Name=B0015
BBa_K598019	.	stem_loop	5712	5841	.	+	0	ID=annotation2144601;Name=B0015
BBa_K598019	.	promoter	5849	5978	.	+	0	ID=annotation2144602;Name=pBAD
BBa_K598019	.	ribosome_entry_site	4565	4571	.	+	0	ID=annotation2144594;Name=rbs
BBa_K598019	.	promoter	138	267	.	+	0	ID=annotation2144595;Name=pBAD
>BBa_K598019
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcg
tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctac
tgtttctccataccgtttttttgggctagcactagaggaggtactagatgaaacattcttccgatatctgcattgttgg
tgctggtatttctggtttgacgtgcgcaagccatctgctggacagcccggcatgccgtggtctgagcctgcgtatcttt
gacatgcagcaagaagccggtggccgtatccgcagcaaaatgctggatggtaaggcaagcattgaactgggcgcaggtc
gctactcccctcagttgcacccgcatttccaaagcgcaatgcagcactatagccaaaagagcgaagtctatccgttcac
ccagttgaagttcaaatctcacgtgcagcaaaagctgaagcgcgccatgaatgaactgtccccgcgtctgaaagagcat
ggtaaagagagctttttgcagtttgtcagccgttatcaaggtcacgatagcgcggttggtatgatccgctctatgggtt
acgacgcactgttcctgccggatatcagcgcagaaatggcctacgacattgtgggtaagcacccggagatccagagcgt
gacggacaacgacgcgaaccaatggtttgcagcggaaacgggctttgctggtctgattcagggcatcaaggctaaggtt
aaggcggcaggtgcgcgttttagcctgggttatcgtctgctgagcgtccgtaccgacggtgacggctacctgctgcaac
tggcaggtgacgacggctggaaactggagcaccgtacccgccatctgattctggcgattccgccgagcgcgatggcggg
tttgaatgttgattttccagaagcctggtccggtgcgcgctatggcagcctgccgctgtttaagggctttctgacgtac
ggtgagccgtggtggttggactacaaactggacgatcaggtgctgattgttgacaacccgctgcgcaaaatctatttca
aaggcgataagtacctgttcttctataccgatagcgagatggcgaattactggcgcggttgtgtcgcggagggcgagga
cggttacctggagcaaattcgcacccatttggctagcgcactgggtatcgtccgtgaacgtatcccgcaaccgctggca
cacgttcacaagtattgggcgcacggcgttgagttttgccgtgattctgatattgaccacccgagcgcactgtctcatc
gcgacagcggtatcatcgcgtgctccgatgcgtacacggagcattgtggttggatggagggcggtctgctgagcgcccg
tgaggcaagccgtctgctgttgcagcgtatcgccgcgtgattaaggaggtaaaaaaaatgagcattctggatttcccgc
gtatccacttccgtggctgggcccgtgtcaatgcgccgaccgcgaaccgcgatccgcacggccacatcgatatggccag
caataccgtggcgatggcgggtgagccgttcgacctggcacgccatcctacggagttccaccgtcacctgcgctccctg
ggtccgcgcttcggcttggatggtcgtgctgacccggaaggcccgttcagcctggccgagggctacaacgctgccggta
acaaccacttttcgtgggagagcgcaaccgttagccacgtgcaatgggatggcggtgaggcggatcgtggtgacggtct
ggtcggtgctcgtttggcactgtggggtcactacaatgattatctgcgtaccaccttcaatcgtgctcgttgggtcgac
agcgacccgacgcgccgtgacgctgcacaaatctatgcgggccaattcaccattagcccggctggtgccggtccgggta
cgccgtggctgtttacggcagacattgatgatagccatggtgcacgttggacgcgtggcggccacattgcagagcgtgg
cggccacttcttggatgaagagtttggtctggcacgcctgtttcagttctctgtgccgaaagatcacccacattttctg
tttcacccgggtccgtttgattccgaggcctggcgtcgtctgcaattggctctggaggatgacgacgttctgggtctga
ccgtgcaatatgcgttgttcaatatgagcaccccgcctcagccgaacagcccggtttttcacgatatggtcggtgttgt
cggtctgtggcgtcgtggtgaactggcgagctacccggctggtcgtctgctgcgtccgcgtcaaccgggtctgggtgac
ctgaccctgcgcgtcaacggtggtcgcgttgcgctgaatttggcgtgtgccattccgttcagcactcgtgccgcgcagc
caagcgcaccggaccgcctgaccccggacctgggtgccaaactgccgctgggcgatctgctgctgcgtgatgaggacgg
cgcactgttggcacgtgtgccgcaggctctgtaccaagactattggacgaatcacggtattgtggacctgccgctgctg
cgcgaaccgcgtggtagcttgaccctgagcagcgaactggcggagtggcgtgagcaagactgggtcacccaaagcgacg
cgtctaacctgtacctggaggcaccggatcgccgtcacggtcgctttttccctgagagcatcgcgctgcgcagctactt
tcgcggtgaagcgcgtgcgcgtccggatatcccgcatcgtatcgagggcatgggcctggtcggcgtcgaatctcgtcag
gatggcgacgctgcggaatggcgtctgacgggtctgcgtccgggtccggcacgcattgttctggacgatggtgccgagg
cgatccctctgcgtgttctgcctgacgattgggcgctggatgacgcgaccgtcgaagaagtggattacgcctttttgta
ccgccacgttatggcgtattacgagctggtgtatccattcatgagcgacaaggtgttttccctggctgatcgttgcaaa
tgtgaaacgtacgcacgtctgatgtggcagatgtgtgatccgcagaaccgcaacaagtcctattacatgccgagcaccc
gcgaactgtcggcaccgaaagctcgtttgttcttgaagtatctggcccacgtggaaggccaggcacgcctgcaagcacc
tccgccagcgggtccggcacgcattgaatctaaagcccagttggcggcagagctgcgtaaagccgtcgacctggagctg
tctgtgatgctgcaatacctgtacgcggcgtatagcattccgaactatgcacagggccaacaacgtgttcgtgacggtg
cgtggaccgccgagcagctgcaactggcgtgcggtagcggtgaccgtcgccgtgatggcggtattcgtgcagcactgct
ggaaattgctcatgaagaaatgattcattacctggtcgttaacaacctgctgatggccctgggcgagccgttctacgcg
ggtgtcccgctgatgggcgaagcggcacgtcaggcgtttggcctggacaccgagttcgctctggaaccgtttagcgaaa
gcacgctggcacgttttgttcgtctggaatggccgcactttatcccagcaccgggcaaatccatcgcggactgctatgc
cgccattcgtcaggcgtttttggatctgccggacttgtttggtggcgaggcaggtaagcgtggcggtgaacaccacctg
ttcctgaatgagctgaccaaccgtgcgcatccgggttatcaactggaagttttcgatcgcgactcggcgctgtttggta
ttgcatttgtgaccgatcagggcgaaggtggcgctctggacagcccgcactacgaacatagccattttcaacgtctgcg
tgaaatgagcgcgcgtatcatggctcaaagcgcaccgttcgaaccggcgctgccggcgttgcgtaatccggttctggat
gagagcccgggttgccaacgtgtcgcagacggtcgtgcgcgtgcgctgatggcattgtaccaaggcgtttatgagctga
tgtttgcgatgatggcgcagcacttcgccgtgaaaccgctgggtagcttgcgtcgcagccgcctgatgaacgcagcaat
cgatctgatgaccggtctgttgcgtccgctgagctgcgcgctgatgaacctgccaagcggcatcgccggtcgcacggcc
ggtccgccgctgccgggtccggttgacacccgtagctatgacgactacgcgctgggctgtcgcatgctggcacgccgtt
gcgagcgtctgctggagcaggcgagcatgctggaaccgggttggctgccggatgcgcagatggagctgctggatttcta
tcgtcgccaaatgctggacttggcgtgcggcaaactgagccgcgaggcctaaggatctttaaggaggtaaaaaaaatga
agattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccat
tgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccg
gcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcata
atgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataa
gtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgat
ctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggcc
gcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatat
ctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgc
gcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacg
gcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggt
tctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctg
ctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgc
agttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcgga
atttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgct
ttgcagcgctgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctga
cgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcactagattctccttcggtacatccag
ctgatgagtcccaaataggacgaaatcctcggggtgcccttctgcgtgaaggctgagaaatacccgtatcacctgatct
ggataatgccagcgtagggatggatcctggattccacgaaggagatataccatggagaaccgtgagccaccactgttgc
cagcccgttggagcagcgcctatgtctcttattggagcccgatgctgccggatgaccagctgaccagcggctattgctg
gttcgactatgaacgtgacatctgtcgtattgacggcctgttcaatccgtggagcgagcgtgatactggttatcgcctg
tggatgtcggaggttggtaatgcggccagcggccgtacctggaaacaaaaagtcgcctatggtcgtgagcgtaccgccc
tgggtgaacagctgtgtgagcgtccgctggatgatgagactggcccttttgccgaattgttcctgccacgcgatgtcct
gcgccgtctgggtgcccgtcacattggccgtcgcgtggttctgggtcgcgaagcggacggttggcgttaccagcgccca
ggtaaaggtccgagcaccctgtacctggatgcggcgagcggcactccactgcgcatggtcaccggcgatgaagcgtcgc
gtgcaagcctgcgtgattttccgaatgtgagcgaggcggagatcccggacgcggttttcgcggccaagcgctaa