LOCUS       BBa_K598022             2945 bp    DNA     linear       26-Dec-2024
DEFINITION  Bistable Switch Mutant 42
ACCESSION   BBa_K598022
VERSION     BBa_K598022.1  
FEATURES             Location/Qualifiers
     CDS             1..681
                     /label=mRFP
     RBS             682..693
                     /label=RBS
     CDS             708..1421
                     /label=lamda CI repressor
     RBS             1422..1433
                     /label=RBS
     promoter        1440..1491
                     /label=PRM (CI434 regulated promoter)
     promoter        1492..1540
                     /label=PR (CI regulated promoter)
     RBS             1556..1567
                     /label=RBS
     CDS             1568..2203
                     /label=CI434 repressor
     RBS             2214..2225
                     /label=RBS
     CDS             2226..2945
                     /label=eGFP
ORIGIN
        1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
       61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
      121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
      181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
      241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
      301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
      361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
      421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
      481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
      541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
      601 gaactcgtga ccgctaacgg aaccttccat acgaactttg aaacgcatga actctttgat
      661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagaatca gccaaacgtc
      721 tcttcaggcc actgactagc gataactttc cccacaacgg aacaactctc attgcatggg
      781 atcattgggt actgtgggtt tagtggttgt aaaaacgcct gaccgctatc cctgatcagt
      841 ttcttgaagg taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca
      901 gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa agcttggctt ggagcctgtt
      961 ggtgcggtca tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg
     1021 gttgtgctta cccatctctc cgcatcacct ttggtaaagg ttctaagctc aggtgagaac
     1081 atccctgcct gaacatgaga aaaaacaggg tactcatgct cacttctaag tgacggctgc
     1141 atactaaccg ctttatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca
     1201 acgctaactt tgagaatttt tgcaagcaat gcggcgttat aagcatttaa tgcattgatg
     1261 ccattaaata aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc
     1321 tgggataagc caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc
     1381 tcaagctgct cttgtgttaa tggtttcttt tttgtgctca tttttttatc agtactagaa
     1441 acaaactttc ttgtacatac gttaaatcta acaaactttc ttgtaaatat ctaacaccgt
     1501 gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa agtacccgca
     1561 gcgttgtatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca
     1621 ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg
     1681 taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga
     1741 ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa
     1801 agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc
     1861 ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg
     1921 attctggctg aaagttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga
     1981 aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc
     2041 caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa
     2101 gtacctgaaa ggcctgaacc cttcatggcc tatgactcct atcaacggaa actgcaagat
     2161 tatcggtgtt gtcgtggaag cgagggtaaa attcgtataa taaactagaa gaggagaaat
     2221 taagcatggt aagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2281 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2341 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2401 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2461 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2521 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2581 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2641 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2701 agagcggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtacagc
     2761 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2821 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2881 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2941 agtaa
//