BBa_K598023 1 BBa_K598023 Bistable Switch with TPP Up-regulated Hammerhead Ribozyme 1.20 Regulating CI434 Expression 2011-09-27T11:00:00Z 2015-05-08T01:12:50Z We construct this part by Gibson Assembly with the bistable switch part from Peking 2007 iGEM team and the part BBa_K598004 The up-regulating TPP ribozyme TPP-1.20 affects the green-red ratio of bistable switch by regulating CI434 expression in this part. false false _770_ 0 8817 9 In stock false We utilize up-regulating TPP ribozyme TPP-1.20 affecting the green-red ratio of bistable switch in order to test our model. false Yan GONG, Xiaowei YAN annotation2150782 1 PRM (CI434 regulated promoter) range2150782 1 1440 1491 annotation2150780 1 lamda CI repressor range2150780 1 708 1421 annotation2150783 1 PR (CI regulated promoter) range2150783 1 1492 1540 annotation2150781 1 RBS range2150781 1 1422 1433 annotation2150779 1 RBS range2150779 1 682 693 annotation2150811 1 native RBS of TPP 1.20 range2150811 1 1682 1694 annotation2150809 1 eGFP range2150809 1 2353 3072 annotation2150807 1 RBS range2150807 1 2341 2352 annotation2150778 1 mRFP range2150778 1 1 681 annotation2150786 1 TPP hammerhead ribozyme 1.20 with native RBS range2150786 1 1545 1694 annotation2150791 1 CI434 repressor range2150791 1 1695 2330 BBa_K598023_sequence 1 ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgctaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagaatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacgcctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatgctcacttctaagtgacggctgcatactaaccgctttatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatttttttatcagtactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcactattctccttcggtacatccagctgatgagtcccaaataggacgaaaacatcggggtgcccttctgcgtgaaggctgagaaatacccgtatcacctgatctggataatgccagcgtagggattattcctggattccacgaaggagatataccatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaagttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaacccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaagaggagaaattaagcatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagagcggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtacagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z