LOCUS       BBa_K598024             3072 bp    DNA     linear       09-May-2024
DEFINITION  Bistable Switch with TPP Down-regulated Hammerhead Ribozyme 2.5 
            Regulating CI434 Expression
ACCESSION   BBa_K598024
VERSION     BBa_K598024.1  
FEATURES             Location/Qualifiers
     CDS             1..681
                     /label=mRFP
     RBS             682..693
                     /label=RBS
     CDS             708..1421
                     /label=lamda CI repressor
     RBS             1422..1433
                     /label=RBS
     promoter        1440..1491
                     /label=PRM (CI434 regulated promoter)
     promoter        1492..1540
                     /label=PR (CI regulated promoter)
     misc_feature    1545..1694
                     /label=TPP hammerhead ribozyme 2.5 with native RBS
     RBS             1682..1694
                     /label=native RBS of TPP 2.5
     CDS             1695..2330
                     /label=CI434 repressor
     RBS             2341..2352
                     /label=RBS
     CDS             2353..3072
                     /label=eGFP
ORIGIN
        1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
       61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
      121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
      181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
      241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
      301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
      361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
      421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
      481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
      541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
      601 gaactcgtga ccgctaacgg aaccttccat acgaactttg aaacgcatga actctttgat
      661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagaatca gccaaacgtc
      721 tcttcaggcc actgactagc gataactttc cccacaacgg aacaactctc attgcatggg
      781 atcattgggt actgtgggtt tagtggttgt aaaaacgcct gaccgctatc cctgatcagt
      841 ttcttgaagg taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca
      901 gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa agcttggctt ggagcctgtt
      961 ggtgcggtca tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg
     1021 gttgtgctta cccatctctc cgcatcacct ttggtaaagg ttctaagctc aggtgagaac
     1081 atccctgcct gaacatgaga aaaaacaggg tactcatgct cacttctaag tgacggctgc
     1141 atactaaccg ctttatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca
     1201 acgctaactt tgagaatttt tgcaagcaat gcggcgttat aagcatttaa tgcattgatg
     1261 ccattaaata aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc
     1321 tgggataagc caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc
     1381 tcaagctgct cttgtgttaa tggtttcttt tttgtgctca tttttttatc agtactagaa
     1441 acaaactttc ttgtacatac gttaaatcta acaaactttc ttgtaaatat ctaacaccgt
     1501 gcgtgttgac tattttacct ctggcggtga taatggttgc actattctcc ttcggtacat
     1561 ccagctgatg agtcccaaat aggacgaaat cctcggggtg cccttctgcg tgaaggctga
     1621 gaaatacccg tatcacctga tctggataat gccagcgtag ggatggatcc tggattccac
     1681 gaaggagata taccatgagt atttcttcca gggtaaaaag caaaagaatc cagcttggac
     1741 ttaaccaggc tgaacttgct caaaaggtgg ggactaccca gcagtctata gagcagctcg
     1801 aaaacggtaa aactaagcga ccacgctttt taccagaact tgcgtcagct cttggcgtaa
     1861 gtgttgactg gctgctcaat ggcacctctg attcgaatgt tagatttgtt gggcacgttg
     1921 agcccaaagg gaaatatcca ttgattagca tggttagagc tggttcgtgg tgtgaagctt
     1981 gtgaacccta cgatatcaag gacattgatg aatggtatga cagtgacgtt aacttattag
     2041 gcaatggatt ctggctgaaa gttgaaggtg attccatgac ctcacctgta ggtcaaagca
     2101 tccctgaagg tcatatggtg ttagtagata ctggacggga gccagtgaat ggaagccttg
     2161 ttgtagccaa actgactgac gcgaacgaag caacattcaa gaaactggtc atagatggcg
     2221 gtcagaagta cctgaaaggc ctgaaccctt catggcctat gactcctatc aacggaaact
     2281 gcaagattat cggtgttgtc gtggaagcga gggtaaaatt cgtataataa actagaagag
     2341 gagaaattaa gcatggtaag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2401 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2461 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2521 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2581 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2641 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2701 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2761 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2821 aagcagaaga gcggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2881 gtacagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2941 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3001 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3061 ctgtacaagt aa
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