LOCUS       BBa_K598025             3116 bp    DNA     linear       09-May-2024
DEFINITION  Bistable Switch with TPP Hammerhead  Ribozyme 2.5  and Theophyline 
            Riboswitch N8-3
ACCESSION   BBa_K598025
VERSION     BBa_K598025.1  
FEATURES             Location/Qualifiers
     CDS             1..681
                     /label=mRFP
     RBS             682..693
                     /label=RBS
     CDS             708..1421
                     /label=lamda CI repressor
     misc_feature    1422..1477
                     /label=Theophyline responsive riboswitch N8-3 with native 
                     RBS
     RBS             1422..1433
                     /label=native RBS of THeo N8-3
     promoter        1484..1535
                     /label=PRM (CI434 regulated promoter)
     misc_feature    1589..1738
                     /label=TPP hammerhead ribozyme 1.20 with native RBS
     promoter        1536..1584
                     /label=PR (CI regulated promoter)
     RBS             1727..1738
                     /label=native RBS of TPP 1.20
     CDS             1739..2374
                     /label=CI434 repressor
     RBS             2385..2396
                     /label=RBS
     CDS             2397..3116
                     /label=eGFP
ORIGIN
        1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
       61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
      121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
      181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
      241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
      301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
      361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
      421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
      481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
      541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
      601 gaactcgtga ccgctaacgg aaccttccat acgaactttg aaacgcatga actctttgat
      661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagaatca gccaaacgtc
      721 tcttcaggcc actgactagc gataactttc cccacaacgg aacaactctc attgcatggg
      781 atcattgggt actgtgggtt tagtggttgt aaaaacgcct gaccgctatc cctgatcagt
      841 ttcttgaagg taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca
      901 gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa agcttggctt ggagcctgtt
      961 ggtgcggtca tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg
     1021 gttgtgctta cccatctctc cgcatcacct ttggtaaagg ttctaagctc aggtgagaac
     1081 atccctgcct gaacatgaga aaaaacaggg tactcatgct cacttctaag tgacggctgc
     1141 atactaaccg ctttatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca
     1201 acgctaactt tgagaatttt tgcaagcaat gcggcgttat aagcatttaa tgcattgatg
     1261 ccattaaata aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc
     1321 tgggataagc caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc
     1381 tcaagctgct cttgtgttaa tggtttcttt tttgtgctca tcttgttgtc tttgcagctg
     1441 gtgctgccaa gggcatcaag acgatgctgg tatcaccact agaaacaaac tttcttgtac
     1501 atacgttaaa tctaacaaac tttcttgtaa atatctaaca ccgtgcgtgt tgactatttt
     1561 acctctggcg gtgataatgg ttgcactatt ctccttcggt acatccagct gatgagtccc
     1621 aaataggacg aaatcctcgg ggtgcccttc tgcgtgaagg ctgagaaata cccgtatcac
     1681 ctgatctgga taatgccagc gtagggatgg atcctggatt ccacgaagga gatataccat
     1741 gagtatttct tccagggtaa aaagcaaaag aatccagctt ggacttaacc aggctgaact
     1801 tgctcaaaag gtggggacta cccagcagtc tatagagcag ctcgaaaacg gtaaaactaa
     1861 gcgaccacgc tttttaccag aacttgcgtc agctcttggc gtaagtgttg actggctgct
     1921 caatggcacc tctgattcga atgttagatt tgttgggcac gttgagccca aagggaaata
     1981 tccattgatt agcatggtta gagctggttc gtggtgtgaa gcttgtgaac cctacgatat
     2041 caaggacatt gatgaatggt atgacagtga cgttaactta ttaggcaatg gattctggct
     2101 gaaagttgaa ggtgattcca tgacctcacc tgtaggtcaa agcatccctg aaggtcatat
     2161 ggtgttagta gatactggac gggagccagt gaatggaagc cttgttgtag ccaaactgac
     2221 tgacgcgaac gaagcaacat tcaagaaact ggtcatagat ggcggtcaga agtacctgaa
     2281 aggcctgaac ccttcatggc ctatgactcc tatcaacgga aactgcaaga ttatcggtgt
     2341 tgtcgtggaa gcgagggtaa aattcgtata ataaactaga agaggagaaa ttaagcatgg
     2401 taagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2461 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2521 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2581 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2641 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2701 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2761 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2821 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagagcggca
     2881 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtacag ctcgccgacc
     2941 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3001 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3061 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaa
//