LOCUS BBa_K598026 5588 bp DNA linear 29-Nov-2024 DEFINITION AND gate (BBa_K228258) regulated by TPP riboswitch 1.20 (BBa_K598004) ACCESSION BBa_K598026 VERSION BBa_K598026.1 FEATURES Location/Qualifiers misc_feature 1..1141 /label=K228008 (AraC generator) CDS 162..1077 /label=AraC misc_feature 1150..1279 /label=I13453 promoter 1150..1279 /label=pBAD misc_feature 1288..1423 /label=SupD tRNA misc_feature 1288..1423 /label=K228001 misc_feature 1432..1560 /label=terminator B0015 CDS 1569..2471 /label=NahR promoter 2550..2620 /label=pSal misc_feature 1568..2620 /label=K228004 misc_feature 2628..2778 /label=TPP riboswitch 1.20 misc_feature 2628..2778 /label=K598004 misc_feature 2778..5451 /label=K228000 CDS 2778..5451 /label=T7ptag misc_feature 5460..5588 /label=terminator B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagtt ctccttcggt 2641 acatccagct gatgagtccc aaataggacg aaaacatcgg ggtgcccttc tgcgtgaagg 2701 ctgagaaata cccgtatcac ctgatctgga taatgccagc gtagggatta ttcctggatt 2761 ccacgaagga gatataccat gaccatgatt accgtgcact agaataccat taacattgct 2821 aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat 2881 tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt 2941 gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac 3001 gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac 3061 tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgtag 3121 gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc 3181 agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac 3241 gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag 3301 gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag 3361 gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa 3421 gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt 3481 agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca 3541 cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg 3601 ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg 3661 gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc 3721 tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca 3781 tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt 3841 ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc 3901 gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag 3961 gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag 4021 tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac 4081 gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg 4141 aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt 4201 gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag 4261 aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct 4321 ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc 4381 tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc 4441 gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt 4501 caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc 4561 aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag 4621 aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc 4681 agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt 4741 caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc 4801 actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg 4861 acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct 4921 gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg 4981 gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg 5041 aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc 5101 gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac 5161 ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt 5221 gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca 5281 gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac 5341 cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa 5401 ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcata atactagagc 5461 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 5521 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 5581 cgtttata //