LOCUS       BBa_K602004             2663 bp    DNA     linear       22-Dec-2024
DEFINITION  PprI expression device
ACCESSION   BBa_K602004
VERSION     BBa_K602004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             207..1664
                     /label=cex coding sequence
     misc_feature    987..992
                     /label=PstI site removed
     CDS             207..1667
                     /label=BBa_K118022
     misc_feature    1674..1676
                     /label=start
     misc_feature    2658..2663
                     /label=stop
     CDS             1674..2663
                     /label=BBa_K602000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatgc ctaggaccac gcccgcaccc ggccacccgg
      241 cccgcggcgc ccgcaccgct ctgcgcacga cgctcgccgc cgcggcggcg acgctcgtcg
      301 tcggcgccac ggtcgtgctg cccgcccagg ccgcgaccac gctcaaggag gccgccgacg
      361 gcgccggccg ggacttcggc ttcgcgctcg accccaaccg gctctcggag gcgcagtaca
      421 aggcgatcgc cgacagcgag ttcaacctcg tcgtcgccga gaacgcgatg aagtgggacg
      481 ccaccgagcc ctcgcagaac agcttctcct tcggcgcggg cgaccgcgtc gcgagctacg
      541 ccgccgacac cggcaaggag ctgtacggcc acacgctcgt atggcactcg cagctgcccg
      601 actgggcgaa gaacctcaac ggctccgcgt tcgagagcgc gatggtcaac cacgtgacga
      661 aggtcgccga ccacttcgag ggcaaggtcg cgtcgtggga cgtcgtcaac gaggcgttcg
      721 ccgacggcgg cggccgccgg caggactcgg cgttccagca gaagctcggc aacggctaca
      781 tcgagaccgc gttccgggcg gcacgtgcgg cggacccgac cgccaagctg tgcatcaacg
      841 actacaacgt cgagggcatc aacgcgaaga gcaactcgct ctacgacctc gtcaaggact
      901 tcaaggcgcg cggcgtcccg ctcgactgcg tcgggttcca gtcgcacctc atcgtcggcc
      961 aggtgccggg cgacttccgg cagaacctgc aacggttcgc ggacctgggc gtggacgtgc
     1021 gcatcaccga gctcgacatc cgcatgcgga cgccctccga cgcgaccaag ctcgcgaccc
     1081 aggcggccga ctacaagaag gtcgtgcagg cctgcatgca ggtgacccgc tgccagggcg
     1141 tgaccgtctg gggcatcacc gacaagtact cgtgggtgcc ggacgtcttc ccgggcgagg
     1201 gggccgcgct ggtgtgggac gcgagctacg ccaagaagcc ggcctacgcc gccgtgatgg
     1261 aggccttcgg cgcgagcccg acgccgacgc ccaccacgcc gaccccgacg cccacgacgc
     1321 cgacgccgac cccgacgtcc ggtccggccg ggtgccaggt gctgtggggc gtcaaccagt
     1381 ggaacaccgg cttcaccgcg aacgtcaccg tgaagaacac gtcctccgct ccggtcgacg
     1441 gctggacgct cacgttcagc ttcccgtccg gccagcaggt cacccaggcg tggagctcga
     1501 cggtcacgca gtccggctcg gccgtgacgg tccgcaacgc cccgtggaac ggctcgatcc
     1561 cggcgggcgg caccgcgcag ttcggcttca acggctcgca cacgggcacc aacgccgcgc
     1621 cgacggcgtt ctcgctcaac ggcacgccct gcacggtcgg ctaataatac tagatgccca
     1681 gtgccaacgt cagcccccct tgcccctctg gggtaagggg cggggggatg gggccaaaag
     1741 ctaaagctga agcctccaag ccccaccccc aaatccctgt taagctccca ttcgtgaccg
     1801 cccccgacgc cctcgccgcc gccaaagcca ggatgcgcga cctggcggcg gcctacgtgg
     1861 cggccctgcc cggacgcgac acccacagcc tgatggcggg ggtgcccggc gtagacctca
     1921 aattcatgcc gctcggctgg cgcgacgggg cgttcgaccc cgagcacaac gtcatcctca
     1981 tcaactcggc ggcccgcccc gaacgccagc gcttcaccct cgcccacgaa atcgggcacg
     2041 cgattttact cggcgacgac gacctgctct ccgacatcca cgacgcctac gagggcgagc
     2101 ggctcgaaca ggtcatcgaa acgctgtgca acgtggcggc ggcggcgatt ttgatgcccg
     2161 aacccgtcat cgcggaaatg ctggaacgct tcggccccac cgggcgcgcc ctcgccgaac
     2221 tcgccaagcg ggccgaagtc agcgcgtcgt cggcgctcta cgccctgacc gagcagaccc
     2281 cggtgcccgt catctacgcg gtctgtgcgc cgggcaagcc tccgcgtgag caggccgcaa
     2341 gcgacgagga cgctggccca agcacagaaa aagtcctgac ggtccgcgcc agcagctcga
     2401 cgcggggcgt caagtacacc ctggcgagcg gcacgccggt acccgccgac cacccggcgg
     2461 cgcttgccct cgccacgggc atggaagtgc gcgaggaaag ctacgtgccc tttcgctcgg
     2521 gccggaaaat gaaggcggag gtggacgcct acccgtcgcg cggcatcgtg gccgtcagtt
     2581 tcgagttcga ccccgcccgc ctgggccgca aggacagcga gcaggccgac cgggacgagc
     2641 cgcaggacgc tgcacagtaa taa
//