BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K602006 1 BBa_K602006 <i>D. radiodurans</i> PprA expression device 2011-10-04T11:00:00Z 2015-05-08T01:12:50Z Standard assembly of the component parts. <i>D. radiodurans</i> PprA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses PprI. Should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>. false false _774_ 0 10901 9 It's complicated true N/A false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh component2152794 1 BBa_B0034 component2152797 1 BBa_K602001 component2152786 1 BBa_R0010 annotation2152797 1 BBa_K602001 range2152797 1 227 1132 annotation2152794 1 BBa_B0034 range2152794 1 209 220 annotation2152786 1 BBa_R0010 range2152786 1 1 200 BBa_K602001 1 BBa_K602001 <i>Deinococcus radiodurans</i> PprA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. dsDNA-binding protein that contributes to the ionizing radiation resistance of <i>Deinococcus radiodurans</i>. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151509 1 start range2151509 1 1 3 annotation2151510 1 stop range2151510 1 901 906 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K602001_sequence 1 atgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K602006_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z