BBa_K602002 1 BBa_K602002 <i>Deinococcus radiodurans</i> PprM 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. Regulatory protein acting downstream of PprI in the DNA damage response of <i>Deinococcus radiodurans</i>. Homolog of cold shock protein (Csp). false false _774_ 0 7851 9 In stock false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151511 1 start range2151511 1 1 3 annotation2151512 1 stop range2151512 1 400 405 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K602009 1 BBa_K602009 <i>D. radiodurans</i> radioresistance genes expression device 2011-10-04T11:00:00Z 2015-05-08T01:12:50Z Standard assembly of component parts. <i>D. radiodurans</i> PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host E. coli. true false _774_ 0 10901 9 Discontinued false N/A false Shao Thing TEOH component2153501 1 BBa_B0034 component2153510 1 BBa_K602002 component2153493 1 BBa_R0010 component2153513 1 BBa_K602003 component2153507 1 BBa_K602001 component2153504 1 BBa_K602000 annotation2153513 1 BBa_K602003 range2153513 1 2546 3640 annotation2153510 1 BBa_K602002 range2153510 1 2135 2539 annotation2153504 1 BBa_K602000 range2153504 1 227 1216 annotation2153493 1 BBa_R0010 range2153493 1 1 200 annotation2153501 1 BBa_B0034 range2153501 1 209 220 annotation2153507 1 BBa_K602001 range2153507 1 1223 2128 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K602003 1 BBa_K602003 <i>Deinococcus radiodurans</i> RecA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. Component of the DNA double-strand break repair mechanism in <i>Deinococcus radiodurans</i>. Binds preferentially to dsDNA even in the presence of ssDNA, unlike <i>E. coli</i> RecA. Essential for radiotolerance of <i>D. radiodurans</i>. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151607 1 stop range2151607 1 1090 1095 annotation2151606 1 start range2151606 1 1 3 BBa_K602001 1 BBa_K602001 <i>Deinococcus radiodurans</i> PprA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. dsDNA-binding protein that contributes to the ionizing radiation resistance of <i>Deinococcus radiodurans</i>. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151509 1 start range2151509 1 1 3 annotation2151510 1 stop range2151510 1 901 906 BBa_K602000 1 BBa_K602000 <i>Deinococcus radiodurans</i> PprI 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. Transcriptional regulator involved in the expression of many DNA damage response proteins in <i>Deinococcus radiodurans</i>. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151508 1 stop range2151508 1 985 990 annotation2151507 1 start range2151507 1 1 3 BBa_K602001_sequence 1 atgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa BBa_K602003_sequence 1 atgagcaaggacgccaccaaagaaatctccgcccccaccgacgccaaggaacgcagcaaggccatcgaaacagccatgagccagatcgaaaaggccttcggcaagggctcgatcatgaaactgggcgccgagagcaaactcgacgtgcaggtcgtcagcaccggcagcctcagccttgacctcgcactgggcgtgggcggcattccgcgtggccgcatcaccgagatctacggccccgagtcgggcggcaagaccaccctggccctcgccatcgtcgcgcaggcccagaaagcgggcggcacctgtgcgtttatcgacgccgagcacgcgctcgacccggtgtacgcccgcgccctgggcgtgaacaccgacgaactgctggtgtcgcagcccgacaacggcgagcaggcgctcgaaatcatggaactgctggtgcgttcgggcgcgattgatgtggtggtcgtggactcggtggctgctctgaccccccgcgccgaaatcgagggcgacatgggcgacagcctgcccggtcttcaggcccgcctgatgtcgcaggcgctgcgcaagctgacggcgattctctccaagaccggcaccgccgccatcttcatcaaccaggttcgcgagaaaatcggcgtgatgtacggcaaccccgaaaccaccaccgggggccgggcgctgaagttctacgccagcgtgcgcctcgacgtgcgtaagatcggccagcccaccaaggtcggcaacgacgcggtcgccaacaccgtcaagatcaagaccgtgaagaacaaggtcgccgcccccttcaaggaagtcgaactcgcgctggtctacggcaagggcttcgaccagctcagcgacctcgtgggcctggccgccgacatggacatcatcaagaaggccggcagcttctactcctacggcgacgagcgcatcggccagggcaaggaaaagaccatcgcctacatcgccgagcgccccgagatggagcaggaaatccgcgaccgcgtgatggccgccatccgcgcgggcaacgcgggcgaagcaccggccctggcccccgcgcctgccgcgcccgaagccgccgaagcgtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K602000_sequence 1 atgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K602002_sequence 1 atgtgccgagtttcgattgttggtggcggtggcccctatcttgcctctcgttcaacaccagcagagaacttgaagggacatgccgggaaaagagcaacagcggcatgtggccttttgttggtgagagcaaggagatttgagatggcaactggaagagtgaagtggttcaacgcggaaaaaggcttcggctttatcgagacggaaggcagcgccgatgtattcgcgcactacagcgcgatcaactcctcgggcttccgcaagctcaacgaaggtgacgaagtcgagttcgaaattgaacccggtcagaacggcaaaggcccccaggccaagaacatcgtggtgaccaaggcagcccccgcgcccgcctatggtgaccgcccccgccgcgacgaccgctggtaataa BBa_K602009_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataatactagatgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataatactagatgtgccgagtttcgattgttggtggcggtggcccctatcttgcctctcgttcaacaccagcagagaacttgaagggacatgccgggaaaagagcaacagcggcatgtggccttttgttggtgagagcaaggagatttgagatggcaactggaagagtgaagtggttcaacgcggaaaaaggcttcggctttatcgagacggaaggcagcgccgatgtattcgcgcactacagcgcgatcaactcctcgggcttccgcaagctcaacgaaggtgacgaagtcgagttcgaaattgaacccggtcagaacggcaaaggcccccaggccaagaacatcgtggtgaccaaggcagcccccgcgcccgcctatggtgaccgcccccgccgcgacgaccgctggtaataatactagatgagcaaggacgccaccaaagaaatctccgcccccaccgacgccaaggaacgcagcaaggccatcgaaacagccatgagccagatcgaaaaggccttcggcaagggctcgatcatgaaactgggcgccgagagcaaactcgacgtgcaggtcgtcagcaccggcagcctcagccttgacctcgcactgggcgtgggcggcattccgcgtggccgcatcaccgagatctacggccccgagtcgggcggcaagaccaccctggccctcgccatcgtcgcgcaggcccagaaagcgggcggcacctgtgcgtttatcgacgccgagcacgcgctcgacccggtgtacgcccgcgccctgggcgtgaacaccgacgaactgctggtgtcgcagcccgacaacggcgagcaggcgctcgaaatcatggaactgctggtgcgttcgggcgcgattgatgtggtggtcgtggactcggtggctgctctgaccccccgcgccgaaatcgagggcgacatgggcgacagcctgcccggtcttcaggcccgcctgatgtcgcaggcgctgcgcaagctgacggcgattctctccaagaccggcaccgccgccatcttcatcaaccaggttcgcgagaaaatcggcgtgatgtacggcaaccccgaaaccaccaccgggggccgggcgctgaagttctacgccagcgtgcgcctcgacgtgcgtaagatcggccagcccaccaaggtcggcaacgacgcggtcgccaacaccgtcaagatcaagaccgtgaagaacaaggtcgccgcccccttcaaggaagtcgaactcgcgctggtctacggcaagggcttcgaccagctcagcgacctcgtgggcctggccgccgacatggacatcatcaagaaggccggcagcttctactcctacggcgacgagcgcatcggccagggcaaggaaaagaccatcgcctacatcgccgagcgccccgagatggagcaggaaatccgcgaccgcgtgatggccgccatccgcgcgggcaacgcgggcgaagcaccggccctggcccccgcgcctgccgcgcccgaagccgccgaagcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z