D. radiodurans radioresistance genes expression device
Standard assembly of the component parts.
D. radiodurans PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host E. coli.
Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
Transcriptional regulator involved in the expression of many DNA damage response proteins in Deinococcus radiodurans.
We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).
dsDNA-binding protein that contributes to the ionizing radiation resistance of Deinococcus radiodurans. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation.
Regulatory protein acting downstream of PprI in the DNA damage response of Deinococcus radiodurans. Homolog of cold shock protein (Csp).
Component of the DNA double-strand break repair mechanism in Deinococcus radiodurans. Binds preferentially to dsDNA even in the presence of ssDNA, unlike E. coli RecA. Essential for radiotolerance of D. radiodurans.
Conversion of the iGEM parts registry to SBOL2.1