BBa_K602011
type: ComponentDefinition
persistent identity: BBa K602011
display id: BBa_K602011
version: 1
was generated by: iGEM to SBOL conversion
description: D. radiodurans radioresistance genes expression device
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Standard assembly of the component parts.
owned by: james
owned by: myers
mutable description: D. radiodurans PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host E. coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _774_
m user id: 0
owner id: 10901
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_K602002
component: BBa_K602003
component: BBa_K602000
component: BBa_K602001
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K602000
sequence annotation: BBa_K602001
sequence annotation: BBa_K602002
sequence annotation: BBa_K602003
sequence: https://synbiohub.org/public/igem/BBa_K602011_sequence/1

BBa_B0034
type: Component
persistent identity: component2154168
display id: component2154168
version: 1
top level: BBa_K602011
definition: BBa_B0034
access: public

BBa_K602002
type: Component
persistent identity: component2154166
display id: component2154166
version: 1
top level: BBa_K602011
definition: BBa_K602002
access: public

BBa_K602003
type: Component
persistent identity: component2154171
display id: component2154171
version: 1
top level: BBa_K602011
definition: BBa_K602003
access: public

BBa_B0034
type: Component
persistent identity: component2154158
display id: component2154158
version: 1
top level: BBa_K602011
definition: BBa_B0034
access: public

BBa_K602000
type: Component
persistent identity: component2154156
display id: component2154156
version: 1
top level: BBa_K602011
definition: BBa_K602000
access: public

BBa_B0034
type: Component
persistent identity: component2154163
display id: component2154163
version: 1
top level: BBa_K602011
definition: BBa_B0034
access: public

BBa_K602001
type: Component
persistent identity: component2154161
display id: component2154161
version: 1
top level: BBa_K602011
definition: BBa_K602001
access: public

BBa_B0034
type: Component
persistent identity: component2154153
display id: component2154153
version: 1
top level: BBa_K602011
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2154145
display id: component2154145
version: 1
top level: BBa_K602011
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2154145
display id: annotation2154145
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154145/range2154145/1
component: BBa_R0010

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type: Range
persistent identity: range2154145
display id: range2154145
version: 1
top level: BBa_K602011
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2154153
display id: annotation2154153
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154153/range2154153/1
component: BBa_B0034

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type: Range
persistent identity: range2154153
display id: range2154153
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K602000
type: SequenceAnnotation
persistent identity: annotation2154156
display id: annotation2154156
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154156/range2154156/1
component: BBa_K602000

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type: Range
persistent identity: range2154156
display id: range2154156
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 227
end: 1216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2154158
display id: annotation2154158
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154158/range2154158/1
component: BBa_B0034

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type: Range
persistent identity: range2154158
display id: range2154158
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 1225
end: 1236
orientation: inline

BBa_K602001
type: SequenceAnnotation
persistent identity: annotation2154161
display id: annotation2154161
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154161/range2154161/1
component: BBa_K602001

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type: Range
persistent identity: range2154161
display id: range2154161
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 1243
end: 2148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2154163
display id: annotation2154163
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154163/range2154163/1
component: BBa_B0034

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type: Range
persistent identity: range2154163
display id: range2154163
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 2157
end: 2168
orientation: inline

BBa_K602002
type: SequenceAnnotation
persistent identity: annotation2154166
display id: annotation2154166
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154166/range2154166/1
component: BBa_K602002

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type: Range
persistent identity: range2154166
display id: range2154166
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 2175
end: 2579
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2154168
display id: annotation2154168
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154168/range2154168/1
component: BBa_B0034

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type: Range
persistent identity: range2154168
display id: range2154168
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 2588
end: 2599
orientation: inline

BBa_K602003
type: SequenceAnnotation
persistent identity: annotation2154171
display id: annotation2154171
version: 1
top level: BBa_K602011
location: https://synbiohub.org/public/igem/BBa_K602011/annotation2154171/range2154171/1
component: BBa_K602003

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type: Range
persistent identity: range2154171
display id: range2154171
version: 1
top level: BBa_K602011
direction: direction/reverse
start: 2606
end: 3700
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K602000
type: ComponentDefinition
persistent identity: BBa K602000
display id: BBa_K602000
version: 1
was generated by: iGEM to SBOL conversion
description: Deinococcus radiodurans PprI
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Obtained by PCR from genomic DNA.
owned by: james
owned by: myers
mutable description: Transcriptional regulator involved in the expression of many DNA damage response proteins in Deinococcus radiodurans.
discontinued: false
dominant: false
experience: experience/Works
group u list: _774_
m user id: 0
owner id: 7851
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).
star: false
creator: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K602000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2151507
display id: annotation2151507
version: 1
top level: BBa_K602000
location: https://synbiohub.org/public/igem/BBa_K602000/annotation2151507/range2151507/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K602000/annotation2151507/range2151507/1
type: Range
persistent identity: range2151507
display id: range2151507
version: 1
top level: BBa_K602000
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2151508
display id: annotation2151508
version: 1
top level: BBa_K602000
location: https://synbiohub.org/public/igem/BBa_K602000/annotation2151508/range2151508/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K602000/annotation2151508/range2151508/1
type: Range
persistent identity: range2151508
display id: range2151508
version: 1
top level: BBa_K602000
direction: direction/forward
start: 985
end: 990
orientation: inline

BBa_K602001
type: ComponentDefinition
persistent identity: BBa K602001
display id: BBa_K602001
version: 1
was generated by: iGEM to SBOL conversion
description: Deinococcus radiodurans PprA
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Obtained by PCR from genomic DNA.
owned by: james
owned by: myers
mutable description: dsDNA-binding protein that contributes to the ionizing radiation resistance of Deinococcus radiodurans. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _774_
m user id: 0
owner id: 7851
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).
star: false
creator: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K602001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2151509
display id: annotation2151509
version: 1
top level: BBa_K602001
location: https://synbiohub.org/public/igem/BBa_K602001/annotation2151509/range2151509/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K602001/annotation2151509/range2151509/1
type: Range
persistent identity: range2151509
display id: range2151509
version: 1
top level: BBa_K602001
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2151510
display id: annotation2151510
version: 1
top level: BBa_K602001
location: https://synbiohub.org/public/igem/BBa_K602001/annotation2151510/range2151510/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K602001/annotation2151510/range2151510/1
type: Range
persistent identity: range2151510
display id: range2151510
version: 1
top level: BBa_K602001
direction: direction/forward
start: 901
end: 906
orientation: inline

BBa_K602002
type: ComponentDefinition
persistent identity: BBa K602002
display id: BBa_K602002
version: 1
was generated by: iGEM to SBOL conversion
description: Deinococcus radiodurans PprM
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Obtained by PCR from genomic DNA.
owned by: james
owned by: myers
mutable description: Regulatory protein acting downstream of PprI in the DNA damage response of Deinococcus radiodurans. Homolog of cold shock protein (Csp).
discontinued: false
dominant: false
experience: experience/Works
group u list: _774_
m user id: 0
owner id: 7851
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).
star: false
creator: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K602002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2151511
display id: annotation2151511
version: 1
top level: BBa_K602002
location: https://synbiohub.org/public/igem/BBa_K602002/annotation2151511/range2151511/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K602002/annotation2151511/range2151511/1
type: Range
persistent identity: range2151511
display id: range2151511
version: 1
top level: BBa_K602002
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2151512
display id: annotation2151512
version: 1
top level: BBa_K602002
location: https://synbiohub.org/public/igem/BBa_K602002/annotation2151512/range2151512/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K602002/annotation2151512/range2151512/1
type: Range
persistent identity: range2151512
display id: range2151512
version: 1
top level: BBa_K602002
direction: direction/forward
start: 400
end: 405
orientation: inline

BBa_K602003
type: ComponentDefinition
persistent identity: BBa K602003
display id: BBa_K602003
version: 1
was generated by: iGEM to SBOL conversion
description: Deinococcus radiodurans RecA
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:50Z
mutable provenance: Obtained by PCR from genomic DNA.
owned by: james
owned by: myers
mutable description: Component of the DNA double-strand break repair mechanism in Deinococcus radiodurans. Binds preferentially to dsDNA even in the presence of ssDNA, unlike E. coli RecA. Essential for radiotolerance of D. radiodurans.
discontinued: false
dominant: false
experience: experience/Works
group u list: _774_
m user id: 0
owner id: 7851
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).
star: false
creator: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K602003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2151606
display id: annotation2151606
version: 1
top level: BBa_K602003
location: https://synbiohub.org/public/igem/BBa_K602003/annotation2151606/range2151606/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K602003/annotation2151606/range2151606/1
type: Range
persistent identity: range2151606
display id: range2151606
version: 1
top level: BBa_K602003
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2151607
display id: annotation2151607
version: 1
top level: BBa_K602003
location: https://synbiohub.org/public/igem/BBa_K602003/annotation2151607/range2151607/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K602003/annotation2151607/range2151607/1
type: Range
persistent identity: range2151607
display id: range2151607
version: 1
top level: BBa_K602003
direction: direction/forward
start: 1090
end: 1095
orientation: inline

https://synbiohub.org/public/igem/BBa_K602011_sequence/1
type: Sequence
persistent identity: BBa K602011 sequence
display id: BBa_K602011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataatactagagaaagaggagaaatactagatgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K602000_sequence/1
type: Sequence
persistent identity: BBa K602000 sequence
display id: BBa_K602000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K602001_sequence/1
type: Sequence
persistent identity: BBa K602001 sequence
display id: BBa_K602001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K602002_sequence/1
type: Sequence
persistent identity: BBa K602002 sequence
display id: BBa_K602002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgccgagtttcgattgttggtggcggtggcccctatcttgcctctcgttcaacaccagcagagaacttgaagggacatgccgggaaaagagcaacagcggcatgtggccttttgttggtgagagcaaggagatttgagatggcaactggaagagtgaagtggttcaacgcggaaaaaggcttcggctttatcgagacggaaggcagcgccgatgtattcgcgcactacagcgcgatcaactcctcgggcttccgcaagctcaacgaaggtgacgaagtcgagttcgaaattgaacccggtcagaacggcaaaggcccccaggccaagaacatcgtggtgaccaaggcagcccccgcgcccgcctatggtgaccgcccccgccgcgacgaccgctggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K602003_sequence/1
type: Sequence
persistent identity: BBa K602003 sequence
display id: BBa_K602003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaaggacgccaccaaagaaatctccgcccccaccgacgccaaggaacgcagcaaggccatcgaaacagccatgagccagatcgaaaaggccttcggcaagggctcgatcatgaaactgggcgccgagagcaaactcgacgtgcaggtcgtcagcaccggcagcctcagccttgacctcgcactgggcgtgggcggcattccgcgtggccgcatcaccgagatctacggccccgagtcgggcggcaagaccaccctggccctcgccatcgtcgcgcaggcccagaaagcgggcggcacctgtgcgtttatcgacgccgagcacgcgctcgacccggtgtacgcccgcgccctgggcgtgaacaccgacgaactgctggtgtcgcagcccgacaacggcgagcaggcgctcgaaatcatggaactgctggtgcgttcgggcgcgattgatgtggtggtcgtggactcggtggctgctctgaccccccgcgccgaaatcgagggcgacatgggcgacagcctgcccggtcttcaggcccgcctgatgtcgcaggcgctgcgcaagctgacggcgattctctccaagaccggcaccgccgccatcttcatcaaccaggttcgcgagaaaatcggcgtgatgtacggcaaccccgaaaccaccaccgggggccgggcgctgaagttctacgccagcgtgcgcctcgacgtgcgtaagatcggccagcccaccaaggtcggcaacgacgcggtcgccaacaccgtcaagatcaagaccgtgaagaacaaggtcgccgcccccttcaaggaagtcgaactcgcgctggtctacggcaagggcttcgaccagctcagcgacctcgtgggcctggccgccgacatggacatcatcaagaaggccggcagcttctactcctacggcgacgagcgcatcggccagggcaaggaaaagaccatcgcctacatcgccgagcgccccgagatggagcaggaaatccgcgaccgcgtgatggccgccatccgcgcgggcaacgcgggcgaagcaccggccctggcccccgcgcctgccgcgcccgaagccgccgaagcgtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z