BBa_K602001 1 BBa_K602001 <i>Deinococcus radiodurans</i> PprA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. dsDNA-binding protein that contributes to the ionizing radiation resistance of <i>Deinococcus radiodurans</i>. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151509 1 start range2151509 1 1 3 annotation2151510 1 stop range2151510 1 901 906 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_K602012 1 BBa_K602012 <i>D. radiodurans</i> PprI-PprA expression device 2011-10-04T11:00:00Z 2015-05-08T01:12:50Z Standard assembly of the component parts. <i>D. radiodurans</i> PprI and PprA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>. true false _774_ 0 10901 9 Discontinued false N/A false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh component2154266 1 BBa_B0034 component2154261 1 BBa_B0034 component2154264 1 BBa_K602000 component2154269 1 BBa_K602001 component2154253 1 BBa_R0010 annotation2154264 1 BBa_K602000 range2154264 1 227 1216 annotation2154261 1 BBa_B0034 range2154261 1 209 220 annotation2154266 1 BBa_B0034 range2154266 1 1225 1236 annotation2154253 1 BBa_R0010 range2154253 1 1 200 annotation2154269 1 BBa_K602001 range2154269 1 1243 2148 BBa_K602000 1 BBa_K602000 <i>Deinococcus radiodurans</i> PprI 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. Transcriptional regulator involved in the expression of many DNA damage response proteins in <i>Deinococcus radiodurans</i>. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151508 1 stop range2151508 1 985 990 annotation2151507 1 start range2151507 1 1 3 BBa_K602001_sequence 1 atgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K602000_sequence 1 atgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K602012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcccagtgccaacgtcagccccccttgcccctctggggtaaggggcggggggatggggccaaaagctaaagctgaagcctccaagccccacccccaaatccctgttaagctcccattcgtgaccgcccccgacgccctcgccgccgccaaagccaggatgcgcgacctggcggcggcctacgtggcggccctgcccggacgcgacacccacagcctgatggcgggggtgcccggcgtagacctcaaattcatgccgctcggctggcgcgacggggcgttcgaccccgagcacaacgtcatcctcatcaactcggcggcccgccccgaacgccagcgcttcaccctcgcccacgaaatcgggcacgcgattttactcggcgacgacgacctgctctccgacatccacgacgcctacgagggcgagcggctcgaacaggtcatcgaaacgctgtgcaacgtggcggcggcggcgattttgatgcccgaacccgtcatcgcggaaatgctggaacgcttcggccccaccgggcgcgccctcgccgaactcgccaagcgggccgaagtcagcgcgtcgtcggcgctctacgccctgaccgagcagaccccggtgcccgtcatctacgcggtctgtgcgccgggcaagcctccgcgtgagcaggccgcaagcgacgaggacgctggcccaagcacagaaaaagtcctgacggtccgcgccagcagctcgacgcggggcgtcaagtacaccctggcgagcggcacgccggtacccgccgaccacccggcggcgcttgccctcgccacgggcatggaagtgcgcgaggaaagctacgtgccctttcgctcgggccggaaaatgaaggcggaggtggacgcctacccgtcgcgcggcatcgtggccgtcagtttcgagttcgaccccgcccgcctgggccgcaaggacagcgagcaggccgaccgggacgagccgcaggacgctgcacagtaataatactagagaaagaggagaaatactagatgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z