LOCUS BBa_K602015 2356 bp DNA linear 22-Dec-2024 DEFINITION D. radiodurans PprI+PprA expression device ACCESSION BBa_K602015 VERSION BBa_K602015.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..229 /label=start misc_feature 1211..1216 /label=stop CDS 227..1216 /label=BBa_K602000 CDS 1225..1312 /label=end of LacI coding region (inactive) misc_feature 1225..1424 /label=BBa_R0010 misc_feature 1313..1350 /label=CAP binding site promoter 1361..1366 /label=-35 promoter 1385..1390 /label=-10 misc_feature 1390..1424 /label=LacI binding site misc_feature 1397..1397 /label=start promoter 1225..1424 /label=BBa_R0010 misc_feature 1437..1440 /label=conserved RBS 1433..1444 /label=BBa_B0034 misc_feature 1451..1453 /label=start misc_feature 2351..2356 /label=stop CDS 1451..2356 /label=BBa_K602001 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc ccagtgccaa 241 cgtcagcccc ccttgcccct ctggggtaag gggcgggggg atggggccaa aagctaaagc 301 tgaagcctcc aagccccacc cccaaatccc tgttaagctc ccattcgtga ccgcccccga 361 cgccctcgcc gccgccaaag ccaggatgcg cgacctggcg gcggcctacg tggcggccct 421 gcccggacgc gacacccaca gcctgatggc gggggtgccc ggcgtagacc tcaaattcat 481 gccgctcggc tggcgcgacg gggcgttcga ccccgagcac aacgtcatcc tcatcaactc 541 ggcggcccgc cccgaacgcc agcgcttcac cctcgcccac gaaatcgggc acgcgatttt 601 actcggcgac gacgacctgc tctccgacat ccacgacgcc tacgagggcg agcggctcga 661 acaggtcatc gaaacgctgt gcaacgtggc ggcggcggcg attttgatgc ccgaacccgt 721 catcgcggaa atgctggaac gcttcggccc caccgggcgc gccctcgccg aactcgccaa 781 gcgggccgaa gtcagcgcgt cgtcggcgct ctacgccctg accgagcaga ccccggtgcc 841 cgtcatctac gcggtctgtg cgccgggcaa gcctccgcgt gagcaggccg caagcgacga 901 ggacgctggc ccaagcacag aaaaagtcct gacggtccgc gccagcagct cgacgcgggg 961 cgtcaagtac accctggcga gcggcacgcc ggtacccgcc gaccacccgg cggcgcttgc 1021 cctcgccacg ggcatggaag tgcgcgagga aagctacgtg ccctttcgct cgggccggaa 1081 aatgaaggcg gaggtggacg cctacccgtc gcgcggcatc gtggccgtca gtttcgagtt 1141 cgaccccgcc cgcctgggcc gcaaggacag cgagcaggcc gaccgggacg agccgcagga 1201 cgctgcacag taataatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga 1261 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 1321 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 1381 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga 1441 gaaatactag atgctacccc tggccttttt aatctgttca gggcataata aaggcagtat 1501 ggcaagggct aaagcaaaag accaaacgga cggcatctac gccgccttcg acaccttgat 1561 gagcacggcg ggcgtggaca gccagatcgc cgccctcgcc gcgagtgagg ccgacgcggg 1621 cacgctggac gcggcgctca cgcagtcctt gcaagaagcg caggggcgct gggggctggg 1681 gctgcaccac ctgcgccatg aggcgcggct gaccgacgac ggcgacatcg aaattctgac 1741 cgatggccgc cccagcgccc gcgtgagcga gggcttcgga gcactcgcgc aggcctacgc 1801 gcccatgcag gcgctcgacg aacgcggcct gagccagtgg gcggcgctcg gcgagggcta 1861 ccgcgctccc ggcgacttgc cgttggcgca gctcaaggtg ctgatcgagc acgcccgcga 1921 cttcgaaacc gactggtcgg cggggcgcgg cgaaaccttt cagcgcgtgt ggcgcaaggg 1981 cgacaccctg tttgtcgagg tggcccggcc cgcgtccgcc gaggccgcgc tctccgacgc 2041 tgcctgggac gtgatcgcca gcatcaagga ccgcgccttc cagcgtgagc tgatgcgccg 2101 cagcgagaag gacgggatgc tcggcgccct gctcggggct cgccacgccg gggccaaggc 2161 caacctcgcc cagctgcccg aagcgcactt caccgtgcag gcgttcgtgc agaccctcag 2221 cggagccgcc gcccgcaacg ccgaggagta ccgcgcggcc ctgaaaaccg ccgccgctgc 2281 gctggaggaa taccagggcg tgaccacccg ccaactgtcc gaagtgctgc ggcacggcct 2341 gcgcgagagc taataa //