LOCUS       BBa_K602016             2545 bp    DNA     linear       22-Nov-2024
DEFINITION  <i>D. radiodurans</i> PprI+RecA expression device
ACCESSION   BBa_K602016
VERSION     BBa_K602016.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1211..1216
                     /label=stop
     CDS             227..1216
                     /label=BBa_K602000
     CDS             1225..1312
                     /label=end of LacI coding region (inactive)
     misc_feature    1225..1424
                     /label=BBa_R0010
     misc_feature    1313..1350
                     /label=CAP binding site
     promoter        1361..1366
                     /label=-35
     promoter        1385..1390
                     /label=-10
     misc_feature    1390..1424
                     /label=LacI binding site
     misc_feature    1397..1397
                     /label=start
     promoter        1225..1424
                     /label=BBa_R0010
     misc_feature    1437..1440
                     /label=conserved
     RBS             1433..1444
                     /label=BBa_B0034
     misc_feature    1451..1453
                     /label=start
     misc_feature    2540..2545
                     /label=stop
     CDS             1451..2545
                     /label=BBa_K602003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc ccagtgccaa
      241 cgtcagcccc ccttgcccct ctggggtaag gggcgggggg atggggccaa aagctaaagc
      301 tgaagcctcc aagccccacc cccaaatccc tgttaagctc ccattcgtga ccgcccccga
      361 cgccctcgcc gccgccaaag ccaggatgcg cgacctggcg gcggcctacg tggcggccct
      421 gcccggacgc gacacccaca gcctgatggc gggggtgccc ggcgtagacc tcaaattcat
      481 gccgctcggc tggcgcgacg gggcgttcga ccccgagcac aacgtcatcc tcatcaactc
      541 ggcggcccgc cccgaacgcc agcgcttcac cctcgcccac gaaatcgggc acgcgatttt
      601 actcggcgac gacgacctgc tctccgacat ccacgacgcc tacgagggcg agcggctcga
      661 acaggtcatc gaaacgctgt gcaacgtggc ggcggcggcg attttgatgc ccgaacccgt
      721 catcgcggaa atgctggaac gcttcggccc caccgggcgc gccctcgccg aactcgccaa
      781 gcgggccgaa gtcagcgcgt cgtcggcgct ctacgccctg accgagcaga ccccggtgcc
      841 cgtcatctac gcggtctgtg cgccgggcaa gcctccgcgt gagcaggccg caagcgacga
      901 ggacgctggc ccaagcacag aaaaagtcct gacggtccgc gccagcagct cgacgcgggg
      961 cgtcaagtac accctggcga gcggcacgcc ggtacccgcc gaccacccgg cggcgcttgc
     1021 cctcgccacg ggcatggaag tgcgcgagga aagctacgtg ccctttcgct cgggccggaa
     1081 aatgaaggcg gaggtggacg cctacccgtc gcgcggcatc gtggccgtca gtttcgagtt
     1141 cgaccccgcc cgcctgggcc gcaaggacag cgagcaggcc gaccgggacg agccgcagga
     1201 cgctgcacag taataatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga
     1261 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     1321 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     1381 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga
     1441 gaaatactag atgagcaagg acgccaccaa agaaatctcc gcccccaccg acgccaagga
     1501 acgcagcaag gccatcgaaa cagccatgag ccagatcgaa aaggccttcg gcaagggctc
     1561 gatcatgaaa ctgggcgccg agagcaaact cgacgtgcag gtcgtcagca ccggcagcct
     1621 cagccttgac ctcgcactgg gcgtgggcgg cattccgcgt ggccgcatca ccgagatcta
     1681 cggccccgag tcgggcggca agaccaccct ggccctcgcc atcgtcgcgc aggcccagaa
     1741 agcgggcggc acctgtgcgt ttatcgacgc cgagcacgcg ctcgacccgg tgtacgcccg
     1801 cgccctgggc gtgaacaccg acgaactgct ggtgtcgcag cccgacaacg gcgagcaggc
     1861 gctcgaaatc atggaactgc tggtgcgttc gggcgcgatt gatgtggtgg tcgtggactc
     1921 ggtggctgct ctgacccccc gcgccgaaat cgagggcgac atgggcgaca gcctgcccgg
     1981 tcttcaggcc cgcctgatgt cgcaggcgct gcgcaagctg acggcgattc tctccaagac
     2041 cggcaccgcc gccatcttca tcaaccaggt tcgcgagaaa atcggcgtga tgtacggcaa
     2101 ccccgaaacc accaccgggg gccgggcgct gaagttctac gccagcgtgc gcctcgacgt
     2161 gcgtaagatc ggccagccca ccaaggtcgg caacgacgcg gtcgccaaca ccgtcaagat
     2221 caagaccgtg aagaacaagg tcgccgcccc cttcaaggaa gtcgaactcg cgctggtcta
     2281 cggcaagggc ttcgaccagc tcagcgacct cgtgggcctg gccgccgaca tggacatcat
     2341 caagaaggcc ggcagcttct actcctacgg cgacgagcgc atcggccagg gcaaggaaaa
     2401 gaccatcgcc tacatcgccg agcgccccga gatggagcag gaaatccgcg accgcgtgat
     2461 ggccgccatc cgcgcgggca acgcgggcga agcaccggcc ctggcccccg cgcctgccgc
     2521 gcccgaagcc gccgaagcgt aataa
//