LOCUS BBa_K602017 2461 bp DNA linear 22-Nov-2024 DEFINITION D. radiodurans PprA+RecA expression device ACCESSION BBa_K602017 VERSION BBa_K602017.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..229 /label=start misc_feature 1127..1132 /label=stop CDS 227..1132 /label=BBa_K602001 CDS 1141..1228 /label=end of LacI coding region (inactive) misc_feature 1141..1340 /label=BBa_R0010 misc_feature 1229..1266 /label=CAP binding site promoter 1277..1282 /label=-35 promoter 1301..1306 /label=-10 misc_feature 1306..1340 /label=LacI binding site misc_feature 1313..1313 /label=start promoter 1141..1340 /label=BBa_R0010 misc_feature 1353..1356 /label=conserved RBS 1349..1360 /label=BBa_B0034 misc_feature 1367..1369 /label=start misc_feature 2456..2461 /label=stop CDS 1367..2461 /label=BBa_K602003 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tacccctggc 241 ctttttaatc tgttcagggc ataataaagg cagtatggca agggctaaag caaaagacca 301 aacggacggc atctacgccg ccttcgacac cttgatgagc acggcgggcg tggacagcca 361 gatcgccgcc ctcgccgcga gtgaggccga cgcgggcacg ctggacgcgg cgctcacgca 421 gtccttgcaa gaagcgcagg ggcgctgggg gctggggctg caccacctgc gccatgaggc 481 gcggctgacc gacgacggcg acatcgaaat tctgaccgat ggccgcccca gcgcccgcgt 541 gagcgagggc ttcggagcac tcgcgcaggc ctacgcgccc atgcaggcgc tcgacgaacg 601 cggcctgagc cagtgggcgg cgctcggcga gggctaccgc gctcccggcg acttgccgtt 661 ggcgcagctc aaggtgctga tcgagcacgc ccgcgacttc gaaaccgact ggtcggcggg 721 gcgcggcgaa acctttcagc gcgtgtggcg caagggcgac accctgtttg tcgaggtggc 781 ccggcccgcg tccgccgagg ccgcgctctc cgacgctgcc tgggacgtga tcgccagcat 841 caaggaccgc gccttccagc gtgagctgat gcgccgcagc gagaaggacg ggatgctcgg 901 cgccctgctc ggggctcgcc acgccggggc caaggccaac ctcgcccagc tgcccgaagc 961 gcacttcacc gtgcaggcgt tcgtgcagac cctcagcgga gccgccgccc gcaacgccga 1021 ggagtaccgc gcggccctga aaaccgccgc cgctgcgctg gaggaatacc agggcgtgac 1081 cacccgccaa ctgtccgaag tgctgcggca cggcctgcgc gagagctaat aatactagag 1141 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 1201 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 1261 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 1321 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcaaggacgc 1381 caccaaagaa atctccgccc ccaccgacgc caaggaacgc agcaaggcca tcgaaacagc 1441 catgagccag atcgaaaagg ccttcggcaa gggctcgatc atgaaactgg gcgccgagag 1501 caaactcgac gtgcaggtcg tcagcaccgg cagcctcagc cttgacctcg cactgggcgt 1561 gggcggcatt ccgcgtggcc gcatcaccga gatctacggc cccgagtcgg gcggcaagac 1621 caccctggcc ctcgccatcg tcgcgcaggc ccagaaagcg ggcggcacct gtgcgtttat 1681 cgacgccgag cacgcgctcg acccggtgta cgcccgcgcc ctgggcgtga acaccgacga 1741 actgctggtg tcgcagcccg acaacggcga gcaggcgctc gaaatcatgg aactgctggt 1801 gcgttcgggc gcgattgatg tggtggtcgt ggactcggtg gctgctctga ccccccgcgc 1861 cgaaatcgag ggcgacatgg gcgacagcct gcccggtctt caggcccgcc tgatgtcgca 1921 ggcgctgcgc aagctgacgg cgattctctc caagaccggc accgccgcca tcttcatcaa 1981 ccaggttcgc gagaaaatcg gcgtgatgta cggcaacccc gaaaccacca ccgggggccg 2041 ggcgctgaag ttctacgcca gcgtgcgcct cgacgtgcgt aagatcggcc agcccaccaa 2101 ggtcggcaac gacgcggtcg ccaacaccgt caagatcaag accgtgaaga acaaggtcgc 2161 cgcccccttc aaggaagtcg aactcgcgct ggtctacggc aagggcttcg accagctcag 2221 cgacctcgtg ggcctggccg ccgacatgga catcatcaag aaggccggca gcttctactc 2281 ctacggcgac gagcgcatcg gccagggcaa ggaaaagacc atcgcctaca tcgccgagcg 2341 ccccgagatg gagcaggaaa tccgcgaccg cgtgatggcc gccatccgcg cgggcaacgc 2401 gggcgaagca ccggccctgg cccccgcgcc tgccgcgccc gaagccgccg aagcgtaata 2461 a //