LOCUS       BBa_K602021             2598 bp    DNA     linear       13-Jun-2024
DEFINITION  placeholder
ACCESSION   BBa_K602021
VERSION     BBa_K602021.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1127..1132
                     /label=stop
     CDS             227..1132
                     /label=BBa_K602001
     stem_loop       1152..1195
                     /label=stem_loop
     misc_feature    1141..1220
                     /label=BBa_B0010
     terminator      1141..1220
                     /label=BBa_B0010
     stem_loop       1236..1255
                     /label=T7 TE
     misc_feature    1262..1262
                     /label=stop
     polyA_site      1256..1269
                     /label=polya
     misc_feature    1229..1269
                     /label=BBa_B0012
     terminator      1229..1269
                     /label=BBa_B0012
     terminator      1141..1269
                     /label=BBa_B0015
     CDS             1278..1365
                     /label=end of LacI coding region (inactive)
     misc_feature    1278..1477
                     /label=BBa_R0010
     misc_feature    1366..1403
                     /label=CAP binding site
     promoter        1414..1419
                     /label=-35
     promoter        1438..1443
                     /label=-10
     misc_feature    1443..1477
                     /label=LacI binding site
     misc_feature    1450..1450
                     /label=start
     promoter        1278..1477
                     /label=BBa_R0010
     misc_feature    1490..1493
                     /label=conserved
     RBS             1486..1497
                     /label=BBa_B0034
     misc_feature    1504..1506
                     /label=start
     misc_feature    2593..2598
                     /label=stop
     CDS             1504..2598
                     /label=BBa_K602003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tacccctggc
      241 ctttttaatc tgttcagggc ataataaagg cagtatggca agggctaaag caaaagacca
      301 aacggacggc atctacgccg ccttcgacac cttgatgagc acggcgggcg tggacagcca
      361 gatcgccgcc ctcgccgcga gtgaggccga cgcgggcacg ctggacgcgg cgctcacgca
      421 gtccttgcaa gaagcgcagg ggcgctgggg gctggggctg caccacctgc gccatgaggc
      481 gcggctgacc gacgacggcg acatcgaaat tctgaccgat ggccgcccca gcgcccgcgt
      541 gagcgagggc ttcggagcac tcgcgcaggc ctacgcgccc atgcaggcgc tcgacgaacg
      601 cggcctgagc cagtgggcgg cgctcggcga gggctaccgc gctcccggcg acttgccgtt
      661 ggcgcagctc aaggtgctga tcgagcacgc ccgcgacttc gaaaccgact ggtcggcggg
      721 gcgcggcgaa acctttcagc gcgtgtggcg caagggcgac accctgtttg tcgaggtggc
      781 ccggcccgcg tccgccgagg ccgcgctctc cgacgctgcc tgggacgtga tcgccagcat
      841 caaggaccgc gccttccagc gtgagctgat gcgccgcagc gagaaggacg ggatgctcgg
      901 cgccctgctc ggggctcgcc acgccggggc caaggccaac ctcgcccagc tgcccgaagc
      961 gcacttcacc gtgcaggcgt tcgtgcagac cctcagcgga gccgccgccc gcaacgccga
     1021 ggagtaccgc gcggccctga aaaccgccgc cgctgcgctg gaggaatacc agggcgtgac
     1081 cacccgccaa ctgtccgaag tgctgcggca cggcctgcgc gagagctaat aatactagag
     1141 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1201 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1261 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     1381 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1441 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac
     1501 tagatgagca aggacgccac caaagaaatc tccgccccca ccgacgccaa ggaacgcagc
     1561 aaggccatcg aaacagccat gagccagatc gaaaaggcct tcggcaaggg ctcgatcatg
     1621 aaactgggcg ccgagagcaa actcgacgtg caggtcgtca gcaccggcag cctcagcctt
     1681 gacctcgcac tgggcgtggg cggcattccg cgtggccgca tcaccgagat ctacggcccc
     1741 gagtcgggcg gcaagaccac cctggccctc gccatcgtcg cgcaggccca gaaagcgggc
     1801 ggcacctgtg cgtttatcga cgccgagcac gcgctcgacc cggtgtacgc ccgcgccctg
     1861 ggcgtgaaca ccgacgaact gctggtgtcg cagcccgaca acggcgagca ggcgctcgaa
     1921 atcatggaac tgctggtgcg ttcgggcgcg attgatgtgg tggtcgtgga ctcggtggct
     1981 gctctgaccc cccgcgccga aatcgagggc gacatgggcg acagcctgcc cggtcttcag
     2041 gcccgcctga tgtcgcaggc gctgcgcaag ctgacggcga ttctctccaa gaccggcacc
     2101 gccgccatct tcatcaacca ggttcgcgag aaaatcggcg tgatgtacgg caaccccgaa
     2161 accaccaccg ggggccgggc gctgaagttc tacgccagcg tgcgcctcga cgtgcgtaag
     2221 atcggccagc ccaccaaggt cggcaacgac gcggtcgcca acaccgtcaa gatcaagacc
     2281 gtgaagaaca aggtcgccgc ccccttcaag gaagtcgaac tcgcgctggt ctacggcaag
     2341 ggcttcgacc agctcagcga cctcgtgggc ctggccgccg acatggacat catcaagaag
     2401 gccggcagct tctactccta cggcgacgag cgcatcggcc agggcaagga aaagaccatc
     2461 gcctacatcg ccgagcgccc cgagatggag caggaaatcc gcgaccgcgt gatggccgcc
     2521 atccgcgcgg gcaacgcggg cgaagcaccg gccctggccc ccgcgcctgc cgcgcccgaa
     2581 gccgccgaag cgtaataa
//