BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K602001 1 BBa_K602001 <i>Deinococcus radiodurans</i> PprA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. dsDNA-binding protein that contributes to the ionizing radiation resistance of <i>Deinococcus radiodurans</i>. Plays a role in DNA repair and genome reconstitution, and is necessary for recovery from genomic fragmentation as a result of exposure to severe levels of ionizing radiation. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151509 1 start range2151509 1 1 3 annotation2151510 1 stop range2151510 1 901 906 BBa_K602003 1 BBa_K602003 <i>Deinococcus radiodurans</i> RecA 2011-10-02T11:00:00Z 2015-05-08T01:12:50Z Obtained by PCR from genomic DNA. Component of the DNA double-strand break repair mechanism in <i>Deinococcus radiodurans</i>. Binds preferentially to dsDNA even in the presence of ssDNA, unlike <i>E. coli</i> RecA. Essential for radiotolerance of <i>D. radiodurans</i>. false false _774_ 0 7851 9 It's complicated false We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10). false Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh annotation2151607 1 stop range2151607 1 1090 1095 annotation2151606 1 start range2151606 1 1 3 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961224 1 -35 range1961224 1 137 142 BBa_K602021 1 BBa_K602021 placeholder 2011-10-27T11:00:00Z 2015-05-08T01:12:50Z placeholder placeholder true false _774_ 0 10901 9 Discontinued false placeholder false Shao Thing TEOH component2161080 1 BBa_K602003 component2161069 1 BBa_R0010 component2161050 1 BBa_R0010 component2161077 1 BBa_B0034 component2161058 1 BBa_B0034 component2161068 1 BBa_B0015 component2161061 1 BBa_K602001 annotation2161068 1 BBa_B0015 range2161068 1 1141 1269 annotation2161050 1 BBa_R0010 range2161050 1 1 200 annotation2161058 1 BBa_B0034 range2161058 1 209 220 annotation2161069 1 BBa_R0010 range2161069 1 1278 1477 annotation2161061 1 BBa_K602001 range2161061 1 227 1132 annotation2161080 1 BBa_K602003 range2161080 1 1504 2598 annotation2161077 1 BBa_B0034 range2161077 1 1486 1497 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K602001_sequence 1 atgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataa BBa_K602003_sequence 1 atgagcaaggacgccaccaaagaaatctccgcccccaccgacgccaaggaacgcagcaaggccatcgaaacagccatgagccagatcgaaaaggccttcggcaagggctcgatcatgaaactgggcgccgagagcaaactcgacgtgcaggtcgtcagcaccggcagcctcagccttgacctcgcactgggcgtgggcggcattccgcgtggccgcatcaccgagatctacggccccgagtcgggcggcaagaccaccctggccctcgccatcgtcgcgcaggcccagaaagcgggcggcacctgtgcgtttatcgacgccgagcacgcgctcgacccggtgtacgcccgcgccctgggcgtgaacaccgacgaactgctggtgtcgcagcccgacaacggcgagcaggcgctcgaaatcatggaactgctggtgcgttcgggcgcgattgatgtggtggtcgtggactcggtggctgctctgaccccccgcgccgaaatcgagggcgacatgggcgacagcctgcccggtcttcaggcccgcctgatgtcgcaggcgctgcgcaagctgacggcgattctctccaagaccggcaccgccgccatcttcatcaaccaggttcgcgagaaaatcggcgtgatgtacggcaaccccgaaaccaccaccgggggccgggcgctgaagttctacgccagcgtgcgcctcgacgtgcgtaagatcggccagcccaccaaggtcggcaacgacgcggtcgccaacaccgtcaagatcaagaccgtgaagaacaaggtcgccgcccccttcaaggaagtcgaactcgcgctggtctacggcaagggcttcgaccagctcagcgacctcgtgggcctggccgccgacatggacatcatcaagaaggccggcagcttctactcctacggcgacgagcgcatcggccagggcaaggaaaagaccatcgcctacatcgccgagcgccccgagatggagcaggaaatccgcgaccgcgtgatggccgccatccgcgcgggcaacgcgggcgaagcaccggccctggcccccgcgcctgccgcgcccgaagccgccgaagcgtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K602021_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctacccctggcctttttaatctgttcagggcataataaaggcagtatggcaagggctaaagcaaaagaccaaacggacggcatctacgccgccttcgacaccttgatgagcacggcgggcgtggacagccagatcgccgccctcgccgcgagtgaggccgacgcgggcacgctggacgcggcgctcacgcagtccttgcaagaagcgcaggggcgctgggggctggggctgcaccacctgcgccatgaggcgcggctgaccgacgacggcgacatcgaaattctgaccgatggccgccccagcgcccgcgtgagcgagggcttcggagcactcgcgcaggcctacgcgcccatgcaggcgctcgacgaacgcggcctgagccagtgggcggcgctcggcgagggctaccgcgctcccggcgacttgccgttggcgcagctcaaggtgctgatcgagcacgcccgcgacttcgaaaccgactggtcggcggggcgcggcgaaacctttcagcgcgtgtggcgcaagggcgacaccctgtttgtcgaggtggcccggcccgcgtccgccgaggccgcgctctccgacgctgcctgggacgtgatcgccagcatcaaggaccgcgccttccagcgtgagctgatgcgccgcagcgagaaggacgggatgctcggcgccctgctcggggctcgccacgccggggccaaggccaacctcgcccagctgcccgaagcgcacttcaccgtgcaggcgttcgtgcagaccctcagcggagccgccgcccgcaacgccgaggagtaccgcgcggccctgaaaaccgccgccgctgcgctggaggaataccagggcgtgaccacccgccaactgtccgaagtgctgcggcacggcctgcgcgagagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcaaggacgccaccaaagaaatctccgcccccaccgacgccaaggaacgcagcaaggccatcgaaacagccatgagccagatcgaaaaggccttcggcaagggctcgatcatgaaactgggcgccgagagcaaactcgacgtgcaggtcgtcagcaccggcagcctcagccttgacctcgcactgggcgtgggcggcattccgcgtggccgcatcaccgagatctacggccccgagtcgggcggcaagaccaccctggccctcgccatcgtcgcgcaggcccagaaagcgggcggcacctgtgcgtttatcgacgccgagcacgcgctcgacccggtgtacgcccgcgccctgggcgtgaacaccgacgaactgctggtgtcgcagcccgacaacggcgagcaggcgctcgaaatcatggaactgctggtgcgttcgggcgcgattgatgtggtggtcgtggactcggtggctgctctgaccccccgcgccgaaatcgagggcgacatgggcgacagcctgcccggtcttcaggcccgcctgatgtcgcaggcgctgcgcaagctgacggcgattctctccaagaccggcaccgccgccatcttcatcaaccaggttcgcgagaaaatcggcgtgatgtacggcaaccccgaaaccaccaccgggggccgggcgctgaagttctacgccagcgtgcgcctcgacgtgcgtaagatcggccagcccaccaaggtcggcaacgacgcggtcgccaacaccgtcaagatcaagaccgtgaagaacaaggtcgccgcccccttcaaggaagtcgaactcgcgctggtctacggcaagggcttcgaccagctcagcgacctcgtgggcctggccgccgacatggacatcatcaagaaggccggcagcttctactcctacggcgacgagcgcatcggccagggcaaggaaaagaccatcgcctacatcgccgagcgccccgagatggagcaggaaatccgcgaccgcgtgatggccgccatccgcgcgggcaacgcgggcgaagcaccggccctggcccccgcgcctgccgcgcccgaagccgccgaagcgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z