LOCUS       BBa_K604003             1524 bp    DNA     linear       22-Nov-2024
DEFINITION  LasI and RFP under Promoter tetR
ACCESSION   BBa_K604003
VERSION     BBa_K604003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..689
                     /label=lasI
     CDS             81..689
                     /label=BBa_C0178
     misc_feature    702..705
                     /label=conserved
     RBS             698..709
                     /label=BBa_B0034
     CDS             716..1390
                     /label=mrfp1
     misc_feature    1397..1421
                     /label=Help:Barcodes
     CDS             716..1421
                     /label=BBa_E1010
     stem_loop       1437..1456
                     /label=T7 TE
     misc_feature    1463..1463
                     /label=stop
     polyA_site      1457..1470
                     /label=polya
     misc_feature    1430..1470
                     /label=BBa_B0012
     terminator      1430..1470
                     /label=BBa_B0012
     stem_loop       1491..1513
                     /label=stem_loop
     misc_feature    1479..1524
                     /label=BBa_B0011
     terminator      1479..1524
                     /label=BBa_B0011
     terminator      1430..1524
                     /label=BBa_B0014
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa
      121 aactgctggg tgaaatgcac aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg
      181 acgtttccgt tatcgacgaa atggaaatcg acggttacga cgctctgtcc ccgtactaca
      241 tgctgatcca ggaagacacc ccggaagctc aggttttcgg ttgctggcgt atcttcgaca
      301 ccaccggtcc gtacatgctg aaaaacacct tcccggaact gctgcacggt aaagaagctc
      361 cgtgctcccc gcacatctgg gaactgtccc gtttcgctat caactccggt cagaaaggtt
      421 ccctgggttt ctccgactgc accctggaag ctatgcgtgc tctggctcgt tactccttgc
      481 agaacgacat ccagaccctg gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc
      541 gtgctggtct ggacgtttcc cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg
      601 ttgctctgcg tatcgaactg aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg
      661 ttgaacagcg tctggctgtt tcctaataat actagagaaa gaggagaaat actagatggc
      721 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      781 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      841 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      901 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      961 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1021 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1081 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1141 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1201 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1261 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1321 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1381 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagt cacactggct
     1441 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt
     1501 attaatccgg cttttttatt attt
//