LOCUS       BBa_K604007             2191 bp    DNA     linear       25-Nov-2024
DEFINITION  autoinducer synthetase rhlI and GFP under PyeaR
ACCESSION   BBa_K604007
VERSION     BBa_K604007.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     misc_feature    113..116
                     /label=conserved
     RBS             109..120
                     /label=BBa_B0034
     CDS             127..729
                     /label=rhlL
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             127..793
                     /label=BBa_C0070
     misc_feature    806..809
                     /label=conserved
     RBS             802..813
                     /label=BBa_B0034
     CDS             823..1439
                     /label=tetR
     CDS             820..1445
                     /label=BBa_C0041
     misc_feature    1458..1461
                     /label=conserved
     RBS             1454..1465
                     /label=BBa_B0034
     CDS             1472..2191
                     /label=GFP protein
     CDS             1472..2191
                     /label=BBa_E0040
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagaa agaggagaaa
      121 tactagatga tcgaactgct gtccgaatcc ctggaaggtc tgtccgctgc tatgatcgct
      181 gaactgggtc gttaccgtca ccaggttttc atcgaaaaac tgggttggga cgttgtttcc
      241 acctcccgtg ttcgtgacca ggagttcgac cagttcgacc acccgcagac ccgttacatc
      301 gttgctatgt cccgtcaggg tatctgcggt tgcgctcgtc tgctgccgac caccgacgct
      361 tacctgctga aagacgtttt cgcttacctg tgctccgaaa ccccgccgtc cgacccgtcc
      421 gtttgggaac tgtcccgtta cgctgcttcc gctgctgacg acccgcagct ggctatgaaa
      481 atcttctggt cctccctcca gtgcgcttgg tacctgggtg cttcctccgt tgttgctgtt
      541 accaccaccg ctatggaacg ttacttcgtt cgtaacggtg ttatcctcca gcgtctgggt
      601 ccgccgcaga aagttaaagg tgaaaccctg gttgctatct ccttcccggc ttaccaggaa
      661 cgtggtctgg aaatgctgct gcgttaccac ccggaatggc tccagggtgt tccgctgtcc
      721 atggctgttg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
      781 tagtgtagat ctctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
      841 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
      901 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
      961 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     1021 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     1081 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     1141 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     1201 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     1261 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     1321 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     1381 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
     1441 aataatacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     1501 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1561 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1621 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     1681 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1741 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1801 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1861 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1921 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1981 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2041 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     2101 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     2161 acatggcatg gatgaactat acaaataata a
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