LOCUS       BBa_K606017             2673 bp    DNA     linear       22-Nov-2024
DEFINITION  T7 polymerase expression casette for Bacillus Subtilis
ACCESSION   BBa_K606017
VERSION     BBa_K606017.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=rbs
     RBS             1..12
                     /label=BBa_K143021
     misc_feature    19..21
                     /label=start
     misc_feature    2668..2670
                     /label=stop
     misc_feature    2671..2673
                     /label=stop
     CDS             22..2667
                     /label=protein
     CDS             19..2673
                     /label=BBa_K145001
ORIGIN
        1 aaaggtggtg aatactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc
       61 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc
      121 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg
      181 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc
      241 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct
      301 aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta
      361 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt
      421 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc
      481 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta
      541 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt
      601 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta
      661 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct
      721 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc
      781 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct
      841 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg
      901 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct
      961 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc
     1021 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg
     1081 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc
     1141 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc
     1201 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc
     1261 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg
     1321 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag
     1381 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg
     1441 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag
     1501 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc
     1561 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg
     1621 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta
     1681 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt
     1741 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta
     1801 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag
     1861 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc
     1921 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc
     1981 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct
     2041 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa
     2101 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag
     2161 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct
     2221 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag
     2281 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag
     2341 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact
     2401 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc
     2461 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac
     2521 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac
     2581 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac
     2641 atcttagagt cggacttcgc gttcgcgtaa taa
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