LOCUS       BBa_K606018             2727 bp    DNA     linear       22-Dec-2024
DEFINITION  T7 poymerase autoamplifier for B. Subtilis
ACCESSION   BBa_K606018
VERSION     BBa_K606018.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             55..66
                     /label=rbs
     RBS             55..66
                     /label=BBa_K143021
     misc_feature    73..75
                     /label=start
     misc_feature    2722..2724
                     /label=stop
     misc_feature    2725..2727
                     /label=stop
     CDS             76..2721
                     /label=protein
     CDS             73..2727
                     /label=BBa_K145001
     misc_feature    55..2727
                     /label=BBa_K606017
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaaggt
       61 ggtgaatact agatgaacac gattaacatc gctaagaacg acttctctga catcgaactg
      121 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag
      181 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag
      241 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc
      301 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc
      361 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac
      421 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct
      481 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac
      541 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac
      601 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc
      661 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc
      721 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta
      781 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc
      841 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag
      901 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg
      961 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg
     1021 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg
     1081 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag
     1141 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg
     1201 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc
     1261 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc
     1321 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt
     1381 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt
     1441 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct
     1501 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca
     1561 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt
     1621 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt
     1681 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt
     1741 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag
     1801 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc
     1861 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg
     1921 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg
     1981 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag
     2041 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac
     2101 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg
     2161 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga
     2221 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg
     2281 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc
     2341 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct
     2401 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg
     2461 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc
     2521 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat
     2581 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct
     2641 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta
     2701 gagtcggact tcgcgttcgc gtaataa
//