LOCUS       BBa_K606022             2048 bp    DNA     linear       10-Dec-2025
DEFINITION  pVeg-SpoVG-GFP-lacI-Term for B. Subtilis
ACCESSION   BBa_K606022
VERSION     BBa_K606022.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..1911
                     /label=cds
     CDS             124..1911
                     /label=BBa_K606020
     misc_feature    1..1911
                     /label=BBa_S04579
     stem_loop       1931..1974
                     /label=stem_loop
     misc_feature    1920..1999
                     /label=BBa_B0010
     terminator      1920..1999
                     /label=BBa_B0010
     stem_loop       2015..2034
                     /label=T7 TE
     misc_feature    2041..2041
                     /label=stop
     polyA_site      2035..2048
                     /label=polya
     misc_feature    2008..2048
                     /label=BBa_B0012
     terminator      2008..2048
                     /label=BBa_B0012
     terminator      1920..2048
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      181 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      241 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      301 cttgtcacta ctttcactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
      361 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      421 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      481 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      541 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      601 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      661 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      721 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      781 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaagaa
      841 tttctgcaac ccgggggatc catggtgaaa ccagtaacgt tatacgatgt cgcagagtat
      901 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      961 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     1021 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     1081 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     1141 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     1201 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     1261 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     1321 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
     1381 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     1441 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     1501 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     1561 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     1621 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     1681 tacgacgata ccgaagacag ctcatgttat atcccgccgt caaccaccat caaacaggat
     1741 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1801 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1861 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtactagagc
     1921 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1981 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2041 cgtttata
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