LOCUS BBa_K606027 1617 bp DNA linear 23-Dec-2024 DEFINITION pVeg-SpoVG-YFP-tetR-Term for B. subtilis and E. coli ACCESSION BBa_K606027 VERSION BBa_K606027.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 CDS 124..1480 /label=YFP:tetR CDS 124..1480 /label=BBa_K606025 misc_feature 1..1480 /label=BBa_S04580 stem_loop 1500..1543 /label=stem_loop misc_feature 1489..1568 /label=BBa_B0010 terminator 1489..1568 /label=BBa_B0010 stem_loop 1584..1603 /label=T7 TE misc_feature 1610..1610 /label=stop polyA_site 1604..1617 /label=polya misc_feature 1577..1617 /label=BBa_B0012 terminator 1577..1617 /label=BBa_B0012 terminator 1489..1617 /label=BBa_B0015 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatggtgt cgagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat 181 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag 241 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag 301 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt 361 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa 421 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc 481 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg 541 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg 601 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat 661 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa 721 aaacaactta aatgtgaaag tgggtctgac atcctcgagt tggtgagcaa gggcgaggag 781 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 841 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 901 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac 961 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 1021 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 1081 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 1141 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 1201 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 1261 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 1321 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc 1381 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 1441 gccgggatca ctctcggcat ggacgagctg tacaagtaat tactagagcc aggcatcaaa 1501 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1561 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //