LOCUS       BBa_K606027             1617 bp    DNA     linear       22-Nov-2024
DEFINITION  pVeg-SpoVG-YFP-tetR-Term for B. subtilis and E. coli
ACCESSION   BBa_K606027
VERSION     BBa_K606027.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..1480
                     /label=YFP:tetR
     CDS             124..1480
                     /label=BBa_K606025
     misc_feature    1..1480
                     /label=BBa_S04580
     stem_loop       1500..1543
                     /label=stem_loop
     misc_feature    1489..1568
                     /label=BBa_B0010
     terminator      1489..1568
                     /label=BBa_B0010
     stem_loop       1584..1603
                     /label=T7 TE
     misc_feature    1610..1610
                     /label=stop
     polyA_site      1604..1617
                     /label=polya
     misc_feature    1577..1617
                     /label=BBa_B0012
     terminator      1577..1617
                     /label=BBa_B0012
     terminator      1489..1617
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatggtgt cgagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
      181 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
      241 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
      301 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
      361 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
      421 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
      481 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
      541 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
      601 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
      661 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
      721 aaacaactta aatgtgaaag tgggtctgac atcctcgagt tggtgagcaa gggcgaggag
      781 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      841 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      901 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
      961 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1021 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1081 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1141 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1201 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1261 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1321 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     1381 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1441 gccgggatca ctctcggcat ggacgagctg tacaagtaat tactagagcc aggcatcaaa
     1501 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1561 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//