LOCUS BBa_K606031 3661 bp DNA linear 22-Dec-2024 DEFINITION Pveg SpoVG T7pol SpoVG GFP double terminator. ACCESSION BBa_K606031 VERSION BBa_K606031.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 misc_feature 124..126 /label=start misc_feature 2773..2775 /label=stop misc_feature 2776..2778 /label=stop CDS 127..2772 /label=protein CDS 124..2778 /label=BBa_K145001 RBS 2787..2798 /label=rbs RBS 2787..2798 /label=BBa_K143021 CDS 2805..3524 /label=GFP protein CDS 2805..3524 /label=BBa_E0040 stem_loop 3544..3587 /label=stem_loop misc_feature 3533..3612 /label=BBa_B0010 terminator 3533..3612 /label=BBa_B0010 stem_loop 3628..3647 /label=T7 TE misc_feature 3654..3654 /label=stop polyA_site 3648..3661 /label=polya misc_feature 3621..3661 /label=BBa_B0012 terminator 3621..3661 /label=BBa_B0012 terminator 3533..3661 /label=BBa_B0015 misc_feature 2787..3524 /label=BBa_K606014 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatgaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc 181 ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt 241 gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt 301 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct 361 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc 421 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt 481 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc 541 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct 601 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag 661 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag 721 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg 781 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa 841 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt 901 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact 961 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact 1021 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg 1081 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac 1141 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa 1201 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt 1261 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag 1321 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac 1381 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg 1441 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg 1501 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc 1561 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac 1621 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct 1681 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct 1741 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt 1801 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac 1861 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat 1921 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa 1981 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac 2041 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt 2101 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag 2161 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt 2221 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt 2281 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac 2341 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct 2401 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct 2461 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac 2521 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct 2581 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt 2641 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac 2701 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac 2761 ttcgcgttcg cgtaataata ctagagaaag gtggtgaata ctagatgcgt aaaggagaag 2821 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 2881 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 2941 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 3001 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 3061 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 3121 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 3181 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 3241 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 3301 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 3361 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 3421 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 3481 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat 3541 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3601 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3661 a //