LOCUS       BBa_K606032             2655 bp    DNA     linear       05-Nov-2024
DEFINITION  T7 RNA polymerase with tyrosine amber codons
ACCESSION   BBa_K606032
VERSION     BBa_K606032.1  
FEATURES             Location/Qualifiers
     CDS             1..2649
                     /label=T7 polymerase
     misc_feature    2650..2652
                     /label=Stop
     misc_feature    2653..2655
                     /label=stop
     misc_feature    84..84
                     /label=Amber mutation
     misc_feature    132..132
                     /label=Amber mutation 2
ORIGIN
        1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg
       61 ttcaacactc tggctgacca ttagggtgag cgtttagctc gcgaacagtt ggcccttgag
      121 catgagtctt aggagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa
      181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag
      241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg
      301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag
      361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca
      421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag
      481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa
      541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg
      601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc
      661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac
      721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg
      781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc
      841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac
      901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt
      961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta
     1021 atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc
     1081 ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct
     1141 gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc
     1201 atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg
     1261 gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc
     1321 aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg
     1381 aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag
     1441 ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact
     1501 tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg
     1561 gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc
     1621 tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac
     1681 ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag
     1741 attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag
     1801 aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg
     1861 ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg
     1921 tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat
     1981 tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg
     2041 atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag
     2101 tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc
     2161 aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag
     2221 aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc
     2281 attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct
     2341 aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag
     2401 aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac
     2461 gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat
     2521 gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa
     2581 atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc
     2641 gcgttcgcgt aataa
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