LOCUS       BBa_K606033             2915 bp    DNA     linear       22-Dec-2024
DEFINITION  pVeg SpoVG T7amber double terminator.
ACCESSION   BBa_K606033
VERSION     BBa_K606033.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..2772
                     /label=T7 polymerase
     misc_feature    2773..2775
                     /label=Stop
     misc_feature    2776..2778
                     /label=stop
     misc_feature    207..207
                     /label=Amber mutation
     misc_feature    255..255
                     /label=Amber mutation 2
     CDS             124..2778
                     /label=BBa_K606032
     stem_loop       2798..2841
                     /label=stem_loop
     misc_feature    2787..2866
                     /label=BBa_B0010
     terminator      2787..2866
                     /label=BBa_B0010
     stem_loop       2882..2901
                     /label=T7 TE
     misc_feature    2908..2908
                     /label=stop
     polyA_site      2902..2915
                     /label=polya
     misc_feature    2875..2915
                     /label=BBa_B0012
     terminator      2875..2915
                     /label=BBa_B0012
     terminator      2787..2915
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc
      181 ccgttcaaca ctctggctga ccattagggt gagcgtttag ctcgcgaaca gttggccctt
      241 gagcatgagt cttaggagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt
      301 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct
      361 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc
      421 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt
      481 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc
      541 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct
      601 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag
      661 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag
      721 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg
      781 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa
      841 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt
      901 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact
      961 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact
     1021 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg
     1081 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac
     1141 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa
     1201 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt
     1261 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag
     1321 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac
     1381 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg
     1441 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg
     1501 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc
     1561 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac
     1621 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct
     1681 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct
     1741 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt
     1801 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac
     1861 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat
     1921 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa
     1981 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac
     2041 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt
     2101 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag
     2161 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt
     2221 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt
     2281 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac
     2341 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct
     2401 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct
     2461 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac
     2521 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct
     2581 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt
     2641 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac
     2701 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac
     2761 ttcgcgttcg cgtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2881 tggctcacct tcgggtgggc ctttctgcgt ttata
//