##gff-version 3
##sequence-region BBa_K606033 1 2915
BBa_K606033	.	engineered_region	1	117	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K606033	.	promoter	1	97	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K606033	.	promoter	63	68	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K606033	.	promoter	86	91	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K606033	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K606033	.	ribosome_entry_site	106	117	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606033	.	CDS	124	2778	.	+	0	ID=BBa_K606032;Name=BBa_K606032
BBa_K606033	.	stop_codon	2773	2775	.	+	0	ID=annotation2132344;Name=Stop;Parent=BBa_K606032
BBa_K606033	.	CDS	124	2772	.	+	0	ID=annotation2132343;Name=T7 polymerase;Parent=BBa_K606032
BBa_K606033	.	sequence_alteration	207	207	.	+	0	ID=annotation2132346;Name=Amber mutation;Parent=BBa_K606032
BBa_K606033	.	sequence_alteration	255	255	.	+	0	ID=annotation2132347;Name=Amber mutation 2;Parent=BBa_K606032
BBa_K606033	.	stop_codon	2776	2778	.	+	0	ID=annotation2132345;Name=stop;Parent=BBa_K606032
BBa_K606033	.	terminator	2787	2915	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K606033	.	terminator	2787	2866	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606033	.	stem_loop	2798	2841	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606033	.	engineered_region	2787	2866	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606033	.	terminator	2875	2915	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606033	.	stop_codon	2908	2908	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606033	.	stem_loop	2882	2901	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606033	.	engineered_region	2875	2915	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606033	.	polyA_site	2902	2915	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K606033
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctc
gtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacacgattaacatcgctaagaacgacttctc
tgacatcgaactggctgctatcccgttcaacactctggctgaccattagggtgagcgtttagctcgcgaacagttggcc
cttgagcatgagtcttaggagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttg
cggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgagga
agtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatc
accattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcggg
ccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaact
caacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactc
ggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagt
caaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctga
atacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcct
aagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagta
agaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatg
gaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcg
attgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctg
ccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataa
gtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaac
ccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggc
tgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaacca
cgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttcctt
gcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggt
cttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtga
aaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgat
aacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctg
gtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagtt
cggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccg
aatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatga
actggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttg
cgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctg
atgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagt
ctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagta
cggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtg
cgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacg
agtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgc
gttcgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata