LOCUS BBa_K606036 3748 bp DNA linear 22-Nov-2024 DEFINITION pT7 SpoVG T7 SpoVG GFP T7terminator. ACCESSION BBa_K606036 VERSION BBa_K606036.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 RBS 32..43 /label=rbs RBS 32..43 /label=BBa_K143021 misc_feature 50..52 /label=start misc_feature 2699..2701 /label=stop misc_feature 2702..2704 /label=stop CDS 53..2698 /label=protein CDS 50..2704 /label=BBa_K145001 RBS 2713..2724 /label=rbs RBS 2713..2724 /label=BBa_K143021 CDS 2731..3450 /label=GFP protein CDS 2731..3450 /label=BBa_E0040 stem_loop 3470..3513 /label=stem_loop misc_feature 3459..3538 /label=BBa_B0010 terminator 3459..3538 /label=BBa_B0010 stem_loop 3554..3573 /label=T7 TE misc_feature 3580..3580 /label=stop polyA_site 3574..3587 /label=polya misc_feature 3547..3587 /label=BBa_B0012 terminator 3547..3587 /label=BBa_B0012 terminator 3459..3587 /label=BBa_B0015 misc_feature 2713..3587 /label=BBa_K606014 misc_feature 3603..3738 /label=J18913 T7ter terminator 3596..3748 /label=BBa_K606028 misc_feature 2713..3748 /label=BBa_K606029 ORIGIN 1 taatacgact cactataggg agatactaga gaaaggtggt gaatactaga tgaacacgat 61 taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct 121 ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta 181 cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt 241 tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg 301 catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca 361 gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc 421 ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc 481 cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa 541 aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca 601 agttgtcgag gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg 661 gcataaggaa gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac 721 cggaatggtt agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat 781 cgaactcgca cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat 841 ctctccgatg ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg 901 tggctattgg gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc 961 actgatgcgc tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca 1021 aaacaccgca tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg 1081 gaagcattgt ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc 1141 ggaagacatc gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt 1201 gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca 1261 agccaataag tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg 1321 tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct 1381 tacgctggcg aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg 1441 tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga 1501 aaaccacgag aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga 1561 gcaagattct ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca 1621 cggcctgagc tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca 1681 gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag 1741 tgaaaccgtt caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc 1801 agacgcaatc aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga 1861 aatctctgag aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg 1921 tgttactcgc agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt 1981 cggcttccgt caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg 2041 tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc 2101 tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa 2161 gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc 2221 tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca 2281 gacgcgcttg aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa 2341 caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca 2401 cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat 2461 cgaatctttt gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct 2521 gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga 2581 tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact 2641 tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcgta 2701 ataatactag agaaaggtgg tgaatactag atgcgtaaag gagaagaact tttcactgga 2761 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 2821 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 2881 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 2941 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 3001 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 3061 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 3121 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 3181 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 3241 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 3301 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 3361 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 3421 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa 3481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtggcc 3601 ggctagtgac tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 3661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 3721 gaggaactat atccggatac cgggttaa //