BBa_K606045 1 BBa_K606045 T7 RNA polymerase double amber 2011-09-17T11:00:00Z 2015-05-08T01:12:51Z The part K145001 has been mutated in two tyrosine site using Site Directed Quick Change mutagenesis. This protein is a T7 RNA polymerase from bacteriophage that have been amber mutated in two distinct tyrosine sites. true false _778_ 0 8998 9 Discontinued false The quick change mutagenesis has been done with a single long primer of 94 bp. So the mutagenesis is quite hard to do. false Cyrille Pauthenier igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z