LOCUS       BBa_K606060              857 bp    DNA     linear       29-Nov-2024
DEFINITION  GFPmut3b tyrosine amber double terminator
ACCESSION   BBa_K606060
VERSION     BBa_K606060.1  
FEATURES             Location/Qualifiers
     misc_feature    198..198
                     /label=amber mutation
     CDS             1..717
                     /label=GFP amber
     misc_feature    718..720
                     /label=stop
     CDS             1..720
                     /label=BBa_K606043
     stem_loop       740..783
                     /label=stem_loop
     misc_feature    729..808
                     /label=BBa_B0010
     terminator      729..808
                     /label=BBa_B0010
     stem_loop       824..843
                     /label=T7 TE
     misc_feature    850..850
                     /label=stop
     polyA_site      844..857
                     /label=polya
     misc_feature    817..857
                     /label=BBa_B0012
     terminator      817..857
                     /label=BBa_B0012
     terminator      729..857
                     /label=BBa_B0015
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttaggg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      781 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      841 gcctttctgc gtttata
//