LOCUS BBa_K606033 2915 bp DNA linear 05-Nov-2024 DEFINITION pVeg SpoVG T7amber double terminator. ACCESSION BBa_K606033 VERSION BBa_K606033.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 CDS 124..2772 /label=T7 polymerase misc_feature 2773..2775 /label=Stop misc_feature 2776..2778 /label=stop misc_feature 207..207 /label=Amber mutation misc_feature 255..255 /label=Amber mutation 2 CDS 124..2778 /label=BBa_K606032 stem_loop 2798..2841 /label=stem_loop misc_feature 2787..2866 /label=BBa_B0010 terminator 2787..2866 /label=BBa_B0010 stem_loop 2882..2901 /label=T7 TE misc_feature 2908..2908 /label=stop polyA_site 2902..2915 /label=polya misc_feature 2875..2915 /label=BBa_B0012 terminator 2875..2915 /label=BBa_B0012 terminator 2787..2915 /label=BBa_B0015 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatgaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc 181 ccgttcaaca ctctggctga ccattagggt gagcgtttag ctcgcgaaca gttggccctt 241 gagcatgagt cttaggagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt 301 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct 361 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc 421 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt 481 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc 541 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct 601 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag 661 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag 721 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg 781 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa 841 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt 901 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact 961 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact 1021 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg 1081 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac 1141 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa 1201 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt 1261 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag 1321 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac 1381 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg 1441 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg 1501 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc 1561 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac 1621 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct 1681 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct 1741 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt 1801 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac 1861 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat 1921 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa 1981 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac 2041 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt 2101 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag 2161 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt 2221 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt 2281 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac 2341 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct 2401 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct 2461 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac 2521 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct 2581 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt 2641 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac 2701 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac 2761 ttcgcgttcg cgtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2881 tggctcacct tcgggtgggc ctttctgcgt ttata // LOCUS BBa_K606062 2915 bp DNA linear 05-Nov-2024 DEFINITION pVeg SopVg T7 RNA amber TT ACCESSION BBa_K606062 VERSION BBa_K606062.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 CDS 124..2772 /label=T7 polymerase misc_feature 2773..2775 /label=Stop misc_feature 2776..2778 /label=stop misc_feature 207..207 /label=Amber mutation misc_feature 255..255 /label=Amber mutation 2 CDS 124..2778 /label=BBa_K606032 stem_loop 2798..2841 /label=stem_loop misc_feature 2787..2866 /label=BBa_B0010 terminator 2787..2866 /label=BBa_B0010 stem_loop 2882..2901 /label=T7 TE misc_feature 2908..2908 /label=stop polyA_site 2902..2915 /label=polya misc_feature 2875..2915 /label=BBa_B0012 terminator 2875..2915 /label=BBa_B0012 terminator 2787..2915 /label=BBa_B0015 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatgaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc 181 ccgttcaaca ctctggctga ccattagggt gagcgtttag ctcgcgaaca gttggccctt 241 gagcatgagt cttaggagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt 301 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct 361 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc 421 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt 481 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc 541 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct 601 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag 661 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag 721 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg 781 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa 841 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt 901 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact 961 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact 1021 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg 1081 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac 1141 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa 1201 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt 1261 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag 1321 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac 1381 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg 1441 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg 1501 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc 1561 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac 1621 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct 1681 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct 1741 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt 1801 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac 1861 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat 1921 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa 1981 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac 2041 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt 2101 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag 2161 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt 2221 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt 2281 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac 2341 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct 2401 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct 2461 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac 2521 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct 2581 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt 2641 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac 2701 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac 2761 ttcgcgttcg cgtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2881 tggctcacct tcgggtgggc ctttctgcgt ttata //