LOCUS BBa_K606063 3990 bp DNA linear 22-Dec-2024 DEFINITION T7 amber expression monitored by a linear system ACCESSION BBa_K606063 VERSION BBa_K606063.1 FEATURES Location/Qualifiers stem_loop 789..832 /label=stem_loop misc_feature 778..857 /label=BBa_B0010 terminator 778..857 /label=BBa_B0010 stem_loop 873..892 /label=T7 TE misc_feature 899..899 /label=stop polyA_site 893..906 /label=polya misc_feature 866..906 /label=BBa_B0012 terminator 866..906 /label=BBa_B0012 promoter 1..23 /label=BBa_I719005 RBS 32..43 /label=rbs RBS 32..43 /label=BBa_K143021 CDS 50..769 /label=GFP protein CDS 50..769 /label=BBa_E0040 stem_loop 789..832 /label=stem_loop misc_feature 778..857 /label=BBa_B0010 terminator 778..857 /label=BBa_B0010 stem_loop 873..892 /label=T7 TE misc_feature 899..899 /label=stop polyA_site 893..906 /label=polya misc_feature 866..906 /label=BBa_B0012 terminator 866..906 /label=BBa_B0012 terminator 778..906 /label=BBa_B0015 misc_feature 32..906 /label=BBa_K606014 misc_feature 922..1057 /label=J18913 T7ter terminator 915..1067 /label=BBa_K606028 misc_feature 32..1067 /label=BBa_K606029 misc_feature 1..1067 /label=BBa_K606035 promoter 1138..1143 /label=Sigma A-35 promoter 1161..1166 /label=Sigma A -10 promoter 1076..1172 /label=BBa_K143012 RBS 1181..1192 /label=rbs RBS 1181..1192 /label=BBa_K143021 misc_feature 1076..1192 /label=BBa_K143053 CDS 1199..3847 /label=T7 polymerase misc_feature 3848..3850 /label=Stop misc_feature 3851..3853 /label=stop misc_feature 1282..1282 /label=Amber mutation misc_feature 1330..1330 /label=Amber mutation 2 CDS 1199..3853 /label=BBa_K606032 stem_loop 3873..3916 /label=stem_loop misc_feature 3862..3941 /label=BBa_B0010 terminator 3862..3941 /label=BBa_B0010 stem_loop 3957..3976 /label=T7 TE misc_feature 3983..3983 /label=stop polyA_site 3977..3990 /label=polya misc_feature 3950..3990 /label=BBa_B0012 terminator 3950..3990 /label=BBa_B0012 terminator 3862..3990 /label=BBa_B0015 misc_feature 1076..3990 /label=BBa_K606033 ORIGIN 1 taatacgact cactataggg agatactaga gaaaggtggt gaatactaga tgcgtaaagg 61 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 121 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 181 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 241 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 301 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 361 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 421 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 481 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 541 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 601 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 661 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 721 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 901 tttatatact agagtggccg gctagtgact gctaacaaag cccgaaagga agctgagttg 961 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg 1021 aggggttttt tgctgaaagg aggaactata tccggatacc gggttaatac tagagaattt 1081 tgtcaaaata attttattga caacgtctta ttaacgttga tataatttaa attttatttg 1141 acaaaaatgg gctcgtgttg tacaataaat gttactagag aaaggtggtg aatactagat 1201 gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt 1261 caacactctg gctgaccatt agggtgagcg tttagctcgc gaacagttgg cccttgagca 1321 tgagtcttag gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc 1381 tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat 1441 gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac 1501 agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac 1561 cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat 1621 cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca 1681 cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc 1741 atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg 1801 gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat 1861 tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc 1921 tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct 1981 ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat 2041 tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag 2101 taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca aagcgattaa 2161 cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat 2221 caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc 2281 gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc 2341 cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat 2401 gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga 2461 ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa 2521 aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact actggctgaa 2581 aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt 2641 cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg 2701 gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt 2761 acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc 2821 tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt 2881 gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag tcaacgagat 2941 tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga ccgatgagaa 3001 cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct 3061 ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc 3121 caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc 3181 cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat 3241 ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc 3301 tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa 3361 gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa 3421 gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac agcctaccat 3481 taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa 3541 ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa 3601 gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc cggctgacgc 3661 tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt 3721 actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat 3781 gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc 3841 gttcgcgtaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3961 caccttcggg tgggcctttc tgcgtttata //