##gff-version 3
##sequence-region BBa_K606063 1 3990
BBa_K606063	.	engineered_region	1076	3990	.	+	0	ID=BBa_K606033;Name=BBa_K606033
BBa_K606063	.	engineered_region	1076	1192	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K606033
BBa_K606063	.	ribosome_entry_site	1181	1192	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K606063	.	ribosome_entry_site	1181	1192	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606063	.	promoter	1076	1172	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K606063	.	promoter	1138	1143	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K606063	.	promoter	1161	1166	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K606063	.	terminator	3862	3990	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606033
BBa_K606063	.	terminator	3862	3941	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606063	.	stem_loop	3873	3916	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606063	.	engineered_region	3862	3941	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606063	.	terminator	3950	3990	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606063	.	stop_codon	3983	3983	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606063	.	polyA_site	3977	3990	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606063	.	stem_loop	3957	3976	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606063	.	engineered_region	3950	3990	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606063	.	CDS	1199	3853	.	+	0	ID=BBa_K606032;Name=BBa_K606032;Parent=BBa_K606033
BBa_K606063	.	stop_codon	3848	3850	.	+	0	ID=annotation2132344;Name=Stop;Parent=BBa_K606032
BBa_K606063	.	stop_codon	3851	3853	.	+	0	ID=annotation2132345;Name=stop;Parent=BBa_K606032
BBa_K606063	.	sequence_alteration	1330	1330	.	+	0	ID=annotation2132347;Name=Amber mutation 2;Parent=BBa_K606032
BBa_K606063	.	CDS	1199	3847	.	+	0	ID=annotation2132343;Name=T7 polymerase;Parent=BBa_K606032
BBa_K606063	.	sequence_alteration	1282	1282	.	+	0	ID=annotation2132346;Name=Amber mutation;Parent=BBa_K606032
BBa_K606063	.	terminator	866	906	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K606063	.	stop_codon	899	899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606063	.	polyA_site	893	906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606063	.	stem_loop	873	892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606063	.	engineered_region	866	906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606063	.	engineered_region	1	1067	.	+	0	ID=BBa_K606035;Name=BBa_K606035
BBa_K606063	.	promoter	1	23	.	+	0	ID=BBa_I719005;Name=BBa_I719005;Parent=BBa_K606035
BBa_K606063	.	engineered_region	32	1067	.	+	0	ID=BBa_K606029;Name=BBa_K606029;Parent=BBa_K606035
BBa_K606063	.	terminator	915	1067	.	+	0	ID=BBa_K606028;Name=BBa_K606028;Parent=BBa_K606029
BBa_K606063	.	stop_codon	922	1057	.	+	0	ID=annotation2131877;Name=J18913 T7ter;Parent=BBa_K606028
BBa_K606063	.	sequence_feature	32	906	.	+	0	ID=BBa_K606014;Name=BBa_K606014;Parent=BBa_K606029
BBa_K606063	.	terminator	778	906	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606014
BBa_K606063	.	terminator	778	857	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606063	.	stem_loop	789	832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606063	.	engineered_region	778	857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606063	.	terminator	866	906	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606063	.	stop_codon	899	899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606063	.	polyA_site	893	906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606063	.	stem_loop	873	892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606063	.	engineered_region	866	906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606063	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K606014
BBa_K606063	.	ribosome_entry_site	32	43	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606063	.	CDS	50	769	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K606014
BBa_K606063	.	CDS	50	769	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K606063	.	terminator	778	857	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K606063	.	stem_loop	789	832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606063	.	engineered_region	778	857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K606063
taatacgactcactatagggagatactagagaaaggtggtgaatactagatgcgtaaaggagaagaacttttcactgga
gttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatg
caacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactac
tttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgccc
gaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaag
gtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgga
atacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattaga
cacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttt
taccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttct
tgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtggccggctagtgactgctaacaaagcccgaaag
gaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggtt
ttttgctgaaaggaggaactatatccggataccgggttaatactagagaattttgtcaaaataattttattgacaacgt
cttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaagg
tggtgaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaac
actctggctgaccattagggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttaggagatgggtgaag
cacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcac
taccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgaca
gccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaacca
gtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtat
ccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaa
gcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataagg
aagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaa
tgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggt
gcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggct
attgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgttta
catgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgcc
aacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccgg
aagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcg
caagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttccct
tacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgc
ttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgt
cgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctcca
ctggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagc
accacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgat
gctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgct
aagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaaca
ctggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcag
tgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagatacc
attcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctga
tttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggc
tgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttc
cctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagccta
ccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagcca
agacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgac
tccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtctt
gtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttcc
ggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata