LOCUS BBa_K606064 6671 bp DNA linear 22-Dec-2024 DEFINITION T7 amber with autoamplification loop ACCESSION BBa_K606064 VERSION BBa_K606064.1 FEATURES Location/Qualifiers stem_loop 3470..3513 /label=stem_loop misc_feature 3459..3538 /label=BBa_B0010 terminator 3459..3538 /label=BBa_B0010 stem_loop 3554..3573 /label=T7 TE misc_feature 3580..3580 /label=stop polyA_site 3574..3587 /label=polya misc_feature 3547..3587 /label=BBa_B0012 terminator 3547..3587 /label=BBa_B0012 promoter 1..23 /label=BBa_I719005 RBS 32..43 /label=rbs RBS 32..43 /label=BBa_K143021 misc_feature 50..52 /label=start misc_feature 2699..2701 /label=stop misc_feature 2702..2704 /label=stop CDS 53..2698 /label=protein CDS 50..2704 /label=BBa_K145001 RBS 2713..2724 /label=rbs RBS 2713..2724 /label=BBa_K143021 CDS 2731..3450 /label=GFP protein CDS 2731..3450 /label=BBa_E0040 stem_loop 3470..3513 /label=stem_loop misc_feature 3459..3538 /label=BBa_B0010 terminator 3459..3538 /label=BBa_B0010 stem_loop 3554..3573 /label=T7 TE misc_feature 3580..3580 /label=stop polyA_site 3574..3587 /label=polya misc_feature 3547..3587 /label=BBa_B0012 terminator 3547..3587 /label=BBa_B0012 terminator 3459..3587 /label=BBa_B0015 misc_feature 2713..3587 /label=BBa_K606014 misc_feature 3603..3738 /label=J18913 T7ter terminator 3596..3748 /label=BBa_K606028 misc_feature 2713..3748 /label=BBa_K606029 misc_feature 1..3748 /label=BBa_K606036 promoter 3819..3824 /label=Sigma A-35 promoter 3842..3847 /label=Sigma A -10 promoter 3757..3853 /label=BBa_K143012 RBS 3862..3873 /label=rbs RBS 3862..3873 /label=BBa_K143021 misc_feature 3757..3873 /label=BBa_K143053 CDS 3880..6528 /label=T7 polymerase misc_feature 6529..6531 /label=Stop misc_feature 6532..6534 /label=stop misc_feature 3963..3963 /label=Amber mutation misc_feature 4011..4011 /label=Amber mutation 2 CDS 3880..6534 /label=BBa_K606032 stem_loop 6554..6597 /label=stem_loop misc_feature 6543..6622 /label=BBa_B0010 terminator 6543..6622 /label=BBa_B0010 stem_loop 6638..6657 /label=T7 TE misc_feature 6664..6664 /label=stop polyA_site 6658..6671 /label=polya misc_feature 6631..6671 /label=BBa_B0012 terminator 6631..6671 /label=BBa_B0012 terminator 6543..6671 /label=BBa_B0015 misc_feature 3757..6671 /label=BBa_K606033 ORIGIN 1 taatacgact cactataggg agatactaga gaaaggtggt gaatactaga tgaacacgat 61 taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct 121 ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta 181 cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt 241 tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg 301 catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca 361 gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc 421 ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc 481 cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa 541 aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca 601 agttgtcgag gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg 661 gcataaggaa gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac 721 cggaatggtt agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat 781 cgaactcgca cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat 841 ctctccgatg ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg 901 tggctattgg gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc 961 actgatgcgc tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca 1021 aaacaccgca tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg 1081 gaagcattgt ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc 1141 ggaagacatc gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt 1201 gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca 1261 agccaataag tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg 1321 tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct 1381 tacgctggcg aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg 1441 tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga 1501 aaaccacgag aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga 1561 gcaagattct ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca 1621 cggcctgagc tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca 1681 gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag 1741 tgaaaccgtt caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc 1801 agacgcaatc aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga 1861 aatctctgag aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg 1921 tgttactcgc agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt 1981 cggcttccgt caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg 2041 tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc 2101 tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa 2161 gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc 2221 tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca 2281 gacgcgcttg aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa 2341 caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca 2401 cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat 2461 cgaatctttt gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct 2521 gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga 2581 tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact 2641 tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcgta 2701 ataatactag agaaaggtgg tgaatactag atgcgtaaag gagaagaact tttcactgga 2761 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 2821 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 2881 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 2941 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 3001 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 3061 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 3121 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 3181 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 3241 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 3301 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 3361 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 3421 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa 3481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtggcc 3601 ggctagtgac tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 3661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 3721 gaggaactat atccggatac cgggttaata ctagagaatt ttgtcaaaat aattttattg 3781 acaacgtctt attaacgttg atataattta aattttattt gacaaaaatg ggctcgtgtt 3841 gtacaataaa tgttactaga gaaaggtggt gaatactaga tgaacacgat taacatcgct 3901 aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat 3961 tagggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta ggagatgggt 4021 gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac 4081 gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac 4141 tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa 4201 gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc 4261 agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac 4321 gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag 4381 gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag 4441 gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa 4501 gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt 4561 agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca 4621 cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg 4681 ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg 4741 gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc 4801 tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca 4861 tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt 4921 ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc 4981 gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag 5041 gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag 5101 tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac 5161 gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg 5221 aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt 5281 gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag 5341 aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct 5401 ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc 5461 tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc 5521 gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt 5581 caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc 5641 aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag 5701 aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc 5761 agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt 5821 caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc 5881 actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg 5941 acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct 6001 gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg 6061 gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg 6121 aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc 6181 gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac 6241 ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt 6301 gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca 6361 gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac 6421 cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa 6481 ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcgta ataatactag 6541 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6601 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6661 ctgcgtttat a //