##gff-version 3
##sequence-region BBa_K606064 1 6671
BBa_K606064	.	engineered_region	3757	6671	.	+	0	ID=BBa_K606033;Name=BBa_K606033
BBa_K606064	.	engineered_region	3757	3873	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K606033
BBa_K606064	.	promoter	3757	3853	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K606064	.	promoter	3819	3824	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K606064	.	promoter	3842	3847	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K606064	.	ribosome_entry_site	3862	3873	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K606064	.	ribosome_entry_site	3862	3873	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606064	.	CDS	3880	6534	.	+	0	ID=BBa_K606032;Name=BBa_K606032;Parent=BBa_K606033
BBa_K606064	.	stop_codon	6532	6534	.	+	0	ID=annotation2132345;Name=stop;Parent=BBa_K606032
BBa_K606064	.	sequence_alteration	4011	4011	.	+	0	ID=annotation2132347;Name=Amber mutation 2;Parent=BBa_K606032
BBa_K606064	.	sequence_alteration	3963	3963	.	+	0	ID=annotation2132346;Name=Amber mutation;Parent=BBa_K606032
BBa_K606064	.	CDS	3880	6528	.	+	0	ID=annotation2132343;Name=T7 polymerase;Parent=BBa_K606032
BBa_K606064	.	stop_codon	6529	6531	.	+	0	ID=annotation2132344;Name=Stop;Parent=BBa_K606032
BBa_K606064	.	terminator	6543	6671	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606033
BBa_K606064	.	terminator	6543	6622	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606064	.	engineered_region	6543	6622	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606064	.	stem_loop	6554	6597	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606064	.	terminator	6631	6671	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606064	.	engineered_region	6631	6671	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606064	.	stop_codon	6664	6664	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606064	.	stem_loop	6638	6657	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606064	.	polyA_site	6658	6671	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606064	.	engineered_region	1	3748	.	+	0	ID=BBa_K606036;Name=BBa_K606036
BBa_K606064	.	engineered_region	2713	3748	.	+	0	ID=BBa_K606029;Name=BBa_K606029;Parent=BBa_K606036
BBa_K606064	.	terminator	3596	3748	.	+	0	ID=BBa_K606028;Name=BBa_K606028;Parent=BBa_K606029
BBa_K606064	.	stop_codon	3603	3738	.	+	0	ID=annotation2131877;Name=J18913 T7ter;Parent=BBa_K606028
BBa_K606064	.	sequence_feature	2713	3587	.	+	0	ID=BBa_K606014;Name=BBa_K606014;Parent=BBa_K606029
BBa_K606064	.	ribosome_entry_site	2713	2724	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K606014
BBa_K606064	.	ribosome_entry_site	2713	2724	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606064	.	CDS	2731	3450	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K606014
BBa_K606064	.	CDS	2731	3450	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K606064	.	terminator	3459	3587	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606014
BBa_K606064	.	terminator	3459	3538	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606064	.	engineered_region	3459	3538	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606064	.	stem_loop	3470	3513	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606064	.	terminator	3547	3587	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606064	.	engineered_region	3547	3587	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606064	.	stop_codon	3580	3580	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606064	.	stem_loop	3554	3573	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606064	.	polyA_site	3574	3587	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606064	.	CDS	50	2704	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K606036
BBa_K606064	.	start_codon	50	52	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K606064	.	stop_codon	2702	2704	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K606064	.	CDS	53	2698	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K606064	.	stop_codon	2699	2701	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K606064	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K606036
BBa_K606064	.	ribosome_entry_site	32	43	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606064	.	promoter	1	23	.	+	0	ID=BBa_I719005;Name=BBa_I719005;Parent=BBa_K606036
BBa_K606064	.	terminator	3547	3587	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K606064	.	engineered_region	3547	3587	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606064	.	stop_codon	3580	3580	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606064	.	stem_loop	3554	3573	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606064	.	polyA_site	3574	3587	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606064	.	terminator	3459	3538	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K606064	.	engineered_region	3459	3538	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606064	.	stem_loop	3470	3513	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K606064
taatacgactcactatagggagatactagagaaaggtggtgaatactagatgaacacgattaacatcgctaagaacgac
ttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagt
tggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtga
ggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggttt
gaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgt
acatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcgg
tcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaa
caactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtc
tactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcat
tgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgca
cctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttc
ctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactca
cagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacacc
gcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatcc
ctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacg
tgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagcc
aataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgt
tcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttacta
ctggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaa
aaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgct
tccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttga
cgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcct
agtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatggga
ccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcact
ggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaa
gagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactc
agccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagc
aatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaag
cgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttga
acctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaaca
ggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgag
aagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaag
cagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagtt
gcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggac
ttcgcgttcgcgtaataatactagagaaaggtggtgaatactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagtggccggctagtgactgctaacaaagcccgaaaggaagc
tgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
ctgaaaggaggaactatatccggataccgggttaatactagagaattttgtcaaaataattttattgacaacgtcttat
taacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtg
aatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactct
ggctgaccattagggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttaggagatgggtgaagcacgc
ttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccc
tactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagcctt
ccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgct
gacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtg
accttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatt
tatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagac
tctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctg
gcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgct
ggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgg
gctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgc
ctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgt
aatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagac
atcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagt
ctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaa
catggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacg
ctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgata
aggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactgga
gaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccac
ggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctcc
gagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaa
agtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggt
gaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtga
ctaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattca
gccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgg
gaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctg
aggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgt
gtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccatt
aacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacg
gtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactcctt
cggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgat
gtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggcta
aaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata