LOCUS       BBa_K606065             1296 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG inducible tRNA Amber emitter with RFP monitor
ACCESSION   BBa_K606065
VERSION     BBa_K606065.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..798
                     /label=mrfp1
     misc_feature    805..829
                     /label=Help:Barcodes
     CDS             124..829
                     /label=BBa_E1010
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     terminator      838..966
                     /label=BBa_B0015
     misc_feature    1..966
                     /label=BBa_K606030
     misc_feature    975..995
                     /label=Lac0
     promoter        1011..1015
                     /label=-35
     promoter        1034..1039
                     /label=-10
     misc_feature    1046..1066
                     /label=LacO
     promoter        975..1066
                     /label=BBa_K606040
     misc_feature    1075..1159
                     /label=BBa_K606034
     stem_loop       1179..1222
                     /label=stem_loop
     misc_feature    1168..1247
                     /label=BBa_B0010
     terminator      1168..1247
                     /label=BBa_B0010
     stem_loop       1263..1282
                     /label=T7 TE
     misc_feature    1289..1289
                     /label=stop
     polyA_site      1283..1296
                     /label=polya
     misc_feature    1256..1296
                     /label=BBa_B0012
     terminator      1256..1296
                     /label=BBa_B0012
     terminator      1168..1296
                     /label=BBa_B0015
     misc_feature    975..1296
                     /label=BBa_K606041
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      181 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      241 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      301 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      361 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      421 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      481 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      541 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      601 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      661 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      721 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      781 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttatatact agagaaatgt gagcactcac aattcatttt gcaaaagttg ttgactttat
     1021 ctacaaggtg tggcataatg tgtgtaattg tgagcggata acaatttact agagggaggg
     1081 gtagcgaagt ggctaaacgc ggcggactct aaatccgctc cctcagggtt cggcagttcg
     1141 aatctgcccc cctccaccat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1261 ctggctcacc ttcgggtggg cctttctgcg tttata
//