LOCUS       BBa_K606066             1676 bp    DNA     linear       22-Dec-2024
DEFINITION  Upstream LacI expression IPTG inducible tRNA emitter
ACCESSION   BBa_K606066
VERSION     BBa_K606066.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..1203
                     /label=LacI (Lva-, N-terminal deletion) regulatory protein
     misc_feature    124..126
                     /label=start
     misc_feature    1204..1206
                     /label=stop
     misc_feature    1207..1209
                     /label=stop
     CDS             124..1209
                     /label=BBa_K143033
     stem_loop       1229..1272
                     /label=stem_loop
     misc_feature    1218..1297
                     /label=BBa_B0010
     terminator      1218..1297
                     /label=BBa_B0010
     stem_loop       1313..1332
                     /label=T7 TE
     misc_feature    1339..1339
                     /label=stop
     polyA_site      1333..1346
                     /label=polya
     misc_feature    1306..1346
                     /label=BBa_B0012
     terminator      1306..1346
                     /label=BBa_B0012
     misc_feature    124..1346
                     /label=BBa_K143062
     misc_feature    1..1346
                     /label=BBa_K606010
     misc_feature    1355..1375
                     /label=Lac0
     promoter        1391..1395
                     /label=-35
     promoter        1414..1419
                     /label=-10
     misc_feature    1426..1446
                     /label=LacO
     promoter        1355..1446
                     /label=BBa_K606040
     misc_feature    1455..1539
                     /label=BBa_K606034
     stem_loop       1559..1602
                     /label=stem_loop
     misc_feature    1548..1627
                     /label=BBa_B0010
     terminator      1548..1627
                     /label=BBa_B0010
     stem_loop       1643..1662
                     /label=T7 TE
     misc_feature    1669..1669
                     /label=stop
     polyA_site      1663..1676
                     /label=polya
     misc_feature    1636..1676
                     /label=BBa_B0012
     terminator      1636..1676
                     /label=BBa_B0012
     terminator      1548..1676
                     /label=BBa_B0015
     misc_feature    1355..1676
                     /label=BBa_K606041
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
      181 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
      241 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
      301 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
      361 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
      421 gaacgaagcg gcgtcgaagc ctgtaaaacg gcggtgcaca atcttctcgc gcaacgcgtc
      481 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
      541 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
      601 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
      661 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
      721 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
      781 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
      841 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
      901 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
      961 tcatgttata tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     1021 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     1081 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     1141 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1201 cagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1261 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1321 ttcgggtggg cctttctgcg tttatatact agagaaatgt gagcactcac aattcatttt
     1381 gcaaaagttg ttgactttat ctacaaggtg tggcataatg tgtgtaattg tgagcggata
     1441 acaatttact agagggaggg gtagcgaagt ggctaaacgc ggcggactct aaatccgctc
     1501 cctcagggtt cggcagttcg aatctgcccc cctccaccat actagagcca ggcatcaaat
     1561 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1621 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//