LacI-pHS-t
type: ComponentDefinition
persistent identity: BBa K606066
display id: BBa_K606066
version: 1
was generated by: iGEM to SBOL conversion
description: Upstream LacI expression IPTG inducible tRNA emitter
created: 2011-10-22T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168
owned by: james
owned by: myers
mutable description: This device are the emitter system for our tRNA diffusion system. It express the tRNA amber who is needed for expression of T7 Amber. In addition a strong LacI expression cassette is added to repress pHyperspank because B. subtilis doesn't express LacI constitutively.
discontinued: false
dominant: false
experience: experience/None
group u list: _778_
m user id: 0
owner id: 8972
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034).
star: false
creator: K??vin Yauy
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K606010
component: BBa_K606041
sequence annotation: BBa_K606010
sequence annotation: BBa_K606041
sequence: https://synbiohub.org/public/igem/BBa_K606066_sequence/1

BBa_K606010
type: Component
persistent identity: component2224576
display id: component2224576
version: 1
top level: LacI-pHS-t
definition: BBa_K606010
access: public

BBa_K606041
type: Component
persistent identity: component2224590
display id: component2224590
version: 1
top level: LacI-pHS-t
definition: pHS-tRNAAm
access: public

BBa_K606010
type: SequenceAnnotation
persistent identity: annotation2224576
display id: annotation2224576
version: 1
top level: LacI-pHS-t
location: https://synbiohub.org/public/igem/BBa_K606066/annotation2224576/range2224576/1
component: BBa_K606010

https://synbiohub.org/public/igem/BBa_K606066/annotation2224576/range2224576/1
type: Range
persistent identity: range2224576
display id: range2224576
version: 1
top level: LacI-pHS-t
direction: direction/reverse
start: 1
end: 1346
orientation: inline

BBa_K606041
type: SequenceAnnotation
persistent identity: annotation2224590
display id: annotation2224590
version: 1
top level: LacI-pHS-t
location: https://synbiohub.org/public/igem/BBa_K606066/annotation2224590/range2224590/1
component: BBa_K606041

https://synbiohub.org/public/igem/BBa_K606066/annotation2224590/range2224590/1
type: Range
persistent identity: range2224590
display id: range2224590
version: 1
top level: LacI-pHS-t
direction: direction/reverse
start: 1355
end: 1676
orientation: inline

BBa_K606010
type: ComponentDefinition
persistent identity: BBa K606010
display id: BBa_K606010
version: 1
was generated by: iGEM to SBOL conversion
description: LacI strong expression casette for B. Subtilis
created: 2011-07-06T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: This part comes from the already existing K143053 and K143062 biobricks
owned by: james
owned by: myers
mutable description: This part is made to produce an expression of LacI in the B. Subtilis cell, in order to control the expression of the promoter pHyperSpank (K143055) level of expression. This is a sub-part of the IPTG inducible expression cassette K606011 for Bacillus Subtilis
discontinued: false
dominant: false
experience: experience/None
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This is just the fusion of the two cassette. The system is in the pSB1C3 E. Coli cloning vector
star: false
creator: Cyrille Pauthenier
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K143062
component: BBa_K143053
sequence annotation: BBa_K143053
sequence annotation: BBa_K143062
sequence: https://synbiohub.org/public/igem/BBa_K606010_sequence/1

BBa_K143062
type: Component
persistent identity: component2123112
display id: component2123112
version: 1
top level: BBa_K606010
definition: LacI-T
access: public

BBa_K143053
type: Component
persistent identity: component2123100
display id: component2123100
version: 1
top level: BBa_K606010
definition: Pveg-spoVG
access: public

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2123100
display id: annotation2123100
version: 1
top level: BBa_K606010
location: https://synbiohub.org/public/igem/BBa_K606010/annotation2123100/range2123100/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K606010/annotation2123100/range2123100/1
type: Range
persistent identity: range2123100
display id: range2123100
version: 1
top level: BBa_K606010
direction: direction/reverse
start: 1
end: 117
orientation: inline

BBa_K143062
type: SequenceAnnotation
persistent identity: annotation2123112
display id: annotation2123112
version: 1
top level: BBa_K606010
location: https://synbiohub.org/public/igem/BBa_K606010/annotation2123112/range2123112/1
component: BBa_K143062

https://synbiohub.org/public/igem/BBa_K606010/annotation2123112/range2123112/1
type: Range
persistent identity: range2123112
display id: range2123112
version: 1
top level: BBa_K606010
direction: direction/reverse
start: 124
end: 1346
orientation: inline

pHS-tRNAAm
type: ComponentDefinition
persistent identity: BBa K606041
display id: BBa_K606041
version: 1
was generated by: iGEM to SBOL conversion
description: pHyperspank Tyrosine amber supressor tRNA double terminator
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: B. subtilis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Tyrosine amber supressor tRNA expression system induced by IPTG in presence of LacI
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: biobrick assembly
star: false
creator: Axel S??guret
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K606034
component: BBa_K606040
sequence annotation: BBa_K606040
sequence annotation: BBa_K606034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K606041_sequence/1

BBa_B0015
type: Component
persistent identity: component2160456
display id: component2160456
version: 1
top level: pHS-tRNAAm
definition: BBa_B0015
access: public

BBa_K606034
type: Component
persistent identity: component2160449
display id: component2160449
version: 1
top level: pHS-tRNAAm
definition: Y taRNA
access: public

BBa_K606040
type: Component
persistent identity: component2160448
display id: component2160448
version: 1
top level: pHS-tRNAAm
definition: pHS
access: public

BBa_K606040
type: SequenceAnnotation
persistent identity: annotation2160448
display id: annotation2160448
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160448/range2160448/1
component: BBa_K606040

https://synbiohub.org/public/igem/BBa_K606041/annotation2160448/range2160448/1
type: Range
persistent identity: range2160448
display id: range2160448
version: 1
top level: pHS-tRNAAm
direction: direction/reverse
start: 1
end: 92
orientation: inline

BBa_K606034
type: SequenceAnnotation
persistent identity: annotation2160449
display id: annotation2160449
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160449/range2160449/1
component: BBa_K606034

https://synbiohub.org/public/igem/BBa_K606041/annotation2160449/range2160449/1
type: Range
persistent identity: range2160449
display id: range2160449
version: 1
top level: pHS-tRNAAm
direction: direction/reverse
start: 101
end: 185
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2160456
display id: annotation2160456
version: 1
top level: pHS-tRNAAm
location: https://synbiohub.org/public/igem/BBa_K606041/annotation2160456/range2160456/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K606041/annotation2160456/range2160456/1
type: Range
persistent identity: range2160456
display id: range2160456
version: 1
top level: pHS-tRNAAm
direction: direction/reverse
start: 194
end: 322
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

LacI-T
type: ComponentDefinition
persistent identity: BBa K143062
display id: BBa_K143062
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor protein - Terminator
created: 2008-09-30T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator.
owned by: james
owned by: myers
mutable description: LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>. The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''. LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator.
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143033
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K143033
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143062_sequence/1

BBa_K143033
type: Component
persistent identity: component1994509
display id: component1994509
version: 1
top level: LacI-T
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1994512
display id: component1994512
version: 1
top level: LacI-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1994510
display id: component1994510
version: 1
top level: LacI-T
definition: BBa_B0010
access: public

BBa_K143033
type: SequenceAnnotation
persistent identity: annotation1994509
display id: annotation1994509
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
component: BBa_K143033

https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
type: Range
persistent identity: range1994509
display id: range1994509
version: 1
top level: LacI-T
direction: direction/reverse
start: 1
end: 1086
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1994510
display id: annotation1994510
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
type: Range
persistent identity: range1994510
display id: range1994510
version: 1
top level: LacI-T
direction: direction/forward
start: 1095
end: 1174
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1994512
display id: annotation1994512
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
type: Range
persistent identity: range1994512
display id: range1994512
version: 1
top level: LacI-T
direction: direction/forward
start: 1183
end: 1223
orientation: inline

Pveg-spoVG
type: ComponentDefinition
persistent identity: BBa K143053
display id: BBa_K143053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS spoVG for B. subtilis
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-spoVG was synthesised by GeneArt
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-spoVG were obtained from papers2 and the sequence synthesised by GeneArt
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143012
component: BBa_K143021
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143053_sequence/1

BBa_K143012
type: Component
persistent identity: component1979395
display id: component1979395
version: 1
top level: Pveg-spoVG
definition: Pveg
access: public

BBa_K143021
type: Component
persistent identity: component1979397
display id: component1979397
version: 1
top level: Pveg-spoVG
definition: RBS-spoVG
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1979395
display id: annotation1979395
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
type: Range
persistent identity: range1979395
display id: range1979395
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1979397
display id: annotation1979397
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
type: Range
persistent identity: range1979397
display id: range1979397
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 106
end: 117
orientation: inline

Y taRNA
type: ComponentDefinition
persistent identity: BBa K606034
display id: BBa_K606034
version: 1
was generated by: iGEM to SBOL conversion
description: Tyrosine amber supressor tRNA
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Tyrosine amber suppressor tRNA for B. Subtilis.
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This sequence is the pure tRNA without the side sequences
star: false
creator: Cyrille Pauthenier
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K606034_sequence/1

pHS
type: ComponentDefinition
persistent identity: BBa K606040
display id: BBa_K606040
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Hyperspank B.subtilis & E.coli
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: inspired from the part BBa_K143015
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: DNA synthesis
star: false
creator: Axel S??guret
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lac0
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K606040_sequence/1

Lac0
type: SequenceAnnotation
persistent identity: annotation2138636
display id: annotation2138636
version: 1
top level: pHS
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138636/range2138636/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K606040/annotation2138636/range2138636/1
type: Range
persistent identity: range2138636
display id: range2138636
version: 1
top level: pHS
direction: direction/forward
start: 1
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2138637
display id: annotation2138637
version: 1
top level: pHS
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138637/range2138637/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K606040/annotation2138637/range2138637/1
type: Range
persistent identity: range2138637
display id: range2138637
version: 1
top level: pHS
direction: direction/forward
start: 37
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2138638
display id: annotation2138638
version: 1
top level: pHS
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138638/range2138638/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K606040/annotation2138638/range2138638/1
type: Range
persistent identity: range2138638
display id: range2138638
version: 1
top level: pHS
direction: direction/forward
start: 60
end: 65
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2138639
display id: annotation2138639
version: 1
top level: pHS
location: https://synbiohub.org/public/igem/BBa_K606040/annotation2138639/range2138639/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K606040/annotation2138639/range2138639/1
type: Range
persistent identity: range2138639
display id: range2138639
version: 1
top level: pHS
direction: direction/forward
start: 72
end: 92
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa K143033
display id: BBa_K143033
version: 1
was generated by: iGEM to SBOL conversion
description: LacI (Lva-, N-terminal deletion) regulatory protein
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
owned by: james
owned by: myers
mutable description: LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars. LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''#1(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output ====References==== <biblio> #1 pmid=16166525 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LacI (Lva-, N-terminal deletion) regulatory protein
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143033_sequence/1

LacI (Lva-, N-terminal deletion) regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975974
display id: annotation1975974
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
type: Range
persistent identity: range1975974
display id: range1975974
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 1080
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992702
display id: annotation1992702
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
type: Range
persistent identity: range1992702
display id: range1992702
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994271
display id: annotation1994271
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
type: Range
persistent identity: range1994271
display id: range1994271
version: 1
top level: LacI
direction: direction/forward
start: 1081
end: 1083
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994272
display id: annotation1994272
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
type: Range
persistent identity: range1994272
display id: range1994272
version: 1
top level: LacI
direction: direction/forward
start: 1084
end: 1086
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

https://synbiohub.org/public/igem/BBa_K606066_sequence/1
type: Sequence
persistent identity: BBa K606066 sequence
display id: BBa_K606066_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatttactagagggaggggtagcgaagtggctaaacgcggcggactctaaatccgctccctcagggttcggcagttcgaatctgcccccctccaccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606010_sequence/1
type: Sequence
persistent identity: BBa K606010 sequence
display id: BBa_K606010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606041_sequence/1
type: Sequence
persistent identity: BBa K606041 sequence
display id: BBa_K606041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatttactagagggaggggtagcgaagtggctaaacgcggcggactctaaatccgctccctcagggttcggcagttcgaatctgcccccctccaccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143062_sequence/1
type: Sequence
persistent identity: BBa K143062 sequence
display id: BBa_K143062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143053_sequence/1
type: Sequence
persistent identity: BBa K143053 sequence
display id: BBa_K143053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606034_sequence/1
type: Sequence
persistent identity: BBa K606034 sequence
display id: BBa_K606034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaggggtagcgaagtggctaaacgcggcggactctaaatccgctccctcagggttcggcagttcgaatctgcccccctccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606040_sequence/1
type: Sequence
persistent identity: BBa K606040 sequence
display id: BBa_K606040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143033_sequence/1
type: Sequence
persistent identity: BBa K143033 sequence
display id: BBa_K143033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z