LOCUS BBa_K606067 2650 bp DNA linear 17-May-2024 DEFINITION Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor ACCESSION BBa_K606067 VERSION BBa_K606067.1 FEATURES Location/Qualifiers promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 promoter 1..97 /label=BBa_K143012 RBS 106..117 /label=rbs RBS 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 CDS 124..1203 /label=LacI (Lva-, N-terminal deletion) regulatory protein misc_feature 124..126 /label=start misc_feature 1204..1206 /label=stop misc_feature 1207..1209 /label=stop CDS 124..1209 /label=BBa_K143033 stem_loop 1229..1272 /label=stem_loop misc_feature 1218..1297 /label=BBa_B0010 terminator 1218..1297 /label=BBa_B0010 stem_loop 1313..1332 /label=T7 TE misc_feature 1339..1339 /label=stop polyA_site 1333..1346 /label=polya misc_feature 1306..1346 /label=BBa_B0012 terminator 1306..1346 /label=BBa_B0012 misc_feature 124..1346 /label=BBa_K143062 misc_feature 1..1346 /label=BBa_K606010 promoter 1417..1422 /label=Sigma A-35 promoter 1440..1445 /label=Sigma A -10 promoter 1355..1451 /label=BBa_K143012 RBS 1460..1471 /label=rbs RBS 1460..1471 /label=BBa_K143021 misc_feature 1355..1471 /label=BBa_K143053 CDS 1478..2152 /label=mrfp1 misc_feature 2159..2183 /label=Help:Barcodes CDS 1478..2183 /label=BBa_E1010 stem_loop 2203..2246 /label=stem_loop misc_feature 2192..2271 /label=BBa_B0010 terminator 2192..2271 /label=BBa_B0010 stem_loop 2287..2306 /label=T7 TE misc_feature 2313..2313 /label=stop polyA_site 2307..2320 /label=polya misc_feature 2280..2320 /label=BBa_B0012 terminator 2280..2320 /label=BBa_B0012 terminator 2192..2320 /label=BBa_B0015 misc_feature 1355..2320 /label=BBa_K606030 misc_feature 2329..2349 /label=Lac0 promoter 2365..2369 /label=-35 promoter 2388..2393 /label=-10 misc_feature 2400..2420 /label=LacO promoter 2329..2420 /label=BBa_K606040 misc_feature 2429..2513 /label=BBa_K606034 stem_loop 2533..2576 /label=stem_loop misc_feature 2522..2601 /label=BBa_B0010 terminator 2522..2601 /label=BBa_B0010 stem_loop 2617..2636 /label=T7 TE misc_feature 2643..2643 /label=stop polyA_site 2637..2650 /label=polya misc_feature 2610..2650 /label=BBa_B0012 terminator 2610..2650 /label=BBa_B0012 terminator 2522..2650 /label=BBa_B0015 misc_feature 2329..2650 /label=BBa_K606041 misc_feature 1355..2650 /label=BBa_K606065 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc 181 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa 241 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa 301 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt 361 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta 421 gaacgaagcg gcgtcgaagc ctgtaaaacg gcggtgcaca atcttctcgc gcaacgcgtc 481 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc 541 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt 601 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac 661 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct 721 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac 781 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc 841 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag 901 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc 961 tcatgttata tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc 1021 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg 1081 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc 1141 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1201 cagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1261 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1321 ttcgggtggg cctttctgcg tttatatact agagaatttt gtcaaaataa ttttattgac 1381 aacgtcttat taacgttgat ataatttaaa ttttatttga caaaaatggg ctcgtgttgt 1441 acaataaatg ttactagaga aaggtggtga atactagatg gcttcctccg aagacgttat 1501 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga 1561 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt 1621 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg 1681 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc 1741 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac 1801 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa 1861 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga 1921 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa 1981 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca 2041 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta 2101 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg 2161 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag 2221 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2281 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa atgtgagcac 2341 tcacaattca ttttgcaaaa gttgttgact ttatctacaa ggtgtggcat aatgtgtgta 2401 attgtgagcg gataacaatt tactagaggg aggggtagcg aagtggctaa acgcggcgga 2461 ctctaaatcc gctccctcag ggttcggcag ttcgaatctg cccccctcca ccatactaga 2521 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2581 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2641 tgcgtttata //