##gff-version 3
##sequence-region BBa_K606067 1 2650
BBa_K606067	.	engineered_region	1	1346	.	+	0	ID=BBa_K606010;Name=BBa_K606010
BBa_K606067	.	engineered_region	1	117	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K606010
BBa_K606067	.	promoter	1	97	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K606067	.	promoter	63	68	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K606067	.	promoter	86	91	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K606067	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K606067	.	ribosome_entry_site	106	117	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606067	.	engineered_region	124	1346	.	+	0	ID=BBa_K143062;Name=BBa_K143062;Parent=BBa_K606010
BBa_K606067	.	terminator	1306	1346	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143062
BBa_K606067	.	engineered_region	1306	1346	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606067	.	stop_codon	1339	1339	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606067	.	polyA_site	1333	1346	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606067	.	stem_loop	1313	1332	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606067	.	terminator	1218	1297	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143062
BBa_K606067	.	stem_loop	1229	1272	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606067	.	engineered_region	1218	1297	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606067	.	CDS	124	1209	.	+	0	ID=BBa_K143033;Name=BBa_K143033;Parent=BBa_K143062
BBa_K606067	.	start_codon	124	126	.	+	0	ID=annotation1992702;Name=start;Parent=BBa_K143033
BBa_K606067	.	stop_codon	1204	1206	.	+	0	ID=annotation1994271;Name=stop;Parent=BBa_K143033
BBa_K606067	.	stop_codon	1207	1209	.	+	0	ID=annotation1994272;Name=stop;Parent=BBa_K143033
BBa_K606067	.	CDS	124	1203	.	+	0	ID=annotation1975974;Name=LacI (Lva-, N-terminal deletion) regulatory protein;Parent=BBa_K143033
BBa_K606067	.	engineered_region	1355	2650	.	+	0	ID=BBa_K606065;Name=BBa_K606065
BBa_K606067	.	engineered_region	2329	2650	.	+	0	ID=BBa_K606041;Name=BBa_K606041;Parent=BBa_K606065
BBa_K606067	.	mature_transcript_region	2429	2513	.	+	0	ID=BBa_K606034;Name=BBa_K606034;Parent=BBa_K606041
BBa_K606067	.	promoter	2329	2420	.	+	0	ID=BBa_K606040;Name=BBa_K606040;Parent=BBa_K606041
BBa_K606067	.	promoter	2365	2369	.	+	0	ID=annotation2138637;Name=-35;Parent=BBa_K606040
BBa_K606067	.	promoter	2388	2393	.	+	0	ID=annotation2138638;Name=-10;Parent=BBa_K606040
BBa_K606067	.	non_covalent_binding_site	2400	2420	.	+	0	ID=annotation2138639;Name=LacO;Parent=BBa_K606040
BBa_K606067	.	non_covalent_binding_site	2329	2349	.	+	0	ID=annotation2138636;Name=Lac0;Parent=BBa_K606040
BBa_K606067	.	terminator	2522	2650	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606041
BBa_K606067	.	terminator	2522	2601	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606067	.	stem_loop	2533	2576	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606067	.	engineered_region	2522	2601	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606067	.	terminator	2610	2650	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606067	.	engineered_region	2610	2650	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606067	.	stop_codon	2643	2643	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606067	.	polyA_site	2637	2650	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606067	.	stem_loop	2617	2636	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606067	.	engineered_region	1355	2320	.	+	0	ID=BBa_K606030;Name=BBa_K606030;Parent=BBa_K606065
BBa_K606067	.	engineered_region	1355	1471	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K606030
BBa_K606067	.	promoter	1355	1451	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K606067	.	promoter	1417	1422	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K606067	.	promoter	1440	1445	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K606067	.	ribosome_entry_site	1460	1471	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K606067	.	ribosome_entry_site	1460	1471	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K606067	.	terminator	2192	2320	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K606030
BBa_K606067	.	terminator	2192	2271	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K606067	.	stem_loop	2203	2246	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K606067	.	engineered_region	2192	2271	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K606067	.	terminator	2280	2320	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K606067	.	engineered_region	2280	2320	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K606067	.	stop_codon	2313	2313	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K606067	.	polyA_site	2307	2320	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K606067	.	stem_loop	2287	2306	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K606067	.	CDS	1478	2183	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K606030
BBa_K606067	.	CDS	1478	2152	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K606067	.	engineered_tag	2159	2183	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K606067
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctc
gtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagta
tgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtg
gaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattg
gcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactggg
tgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaa
cgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttc
cggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactggg
cgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctg
cgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgcca
tgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagat
ggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgat
accgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtgg
accgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaac
caccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcc
cgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgaca
aaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagaaatgtgagcactcacaattcattttgcaaaagttgttgact
ttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatttactagagggaggggtagcgaagtggcta
aacgcggcggactctaaatccgctccctcagggttcggcagttcgaatctgcccccctccaccatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata