LOCUS       BBa_I13501               843 bp    DNA     linear       27-Dec-2024
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13501
VERSION     BBa_I13501.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 ata
//
LOCUS       BBa_K606068             1686 bp    DNA     linear       27-Dec-2024
DEFINITION  pVeg SpoVG cI SpoVG RFP Term for B. subtilis and E. coli
ACCESSION   BBa_K606068
VERSION     BBa_K606068.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..792
                     /label=cI 434
     misc_feature    754..792
                     /label=LVA
     misc_feature    124..792
                     /label=BBa_C0052
     misc_feature    793..817
                     /label=Help:Barcodes
     CDS             124..792
                     /label=BBa_C0052
     misc_feature    1..817
                     /label=BBa_K606055
     RBS             826..837
                     /label=rbs
     RBS             826..837
                     /label=BBa_K143021
     CDS             844..1518
                     /label=mrfp1
     misc_feature    1525..1549
                     /label=Help:Barcodes
     CDS             844..1524
                     /label=BBa_E1010
     stem_loop       1569..1612
                     /label=stem_loop
     misc_feature    1558..1637
                     /label=BBa_B0010
     terminator      1558..1637
                     /label=BBa_B0010
     stem_loop       1653..1672
                     /label=T7 TE
     misc_feature    1679..1679
                     /label=stop
     polyA_site      1673..1686
                     /label=polya
     misc_feature    1646..1686
                     /label=BBa_B0012
     terminator      1646..1686
                     /label=BBa_B0012
     terminator      1558..1686
                     /label=BBa_B0015
     CDS             844..1686
                     /label=BBa_K606012
     misc_feature    826..1686
                     /label=BBa_K606013
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgagta tttcttccag ggtaaaaagc aaaagaatcc agcttggact taaccaggct
      181 gaacttgctc aaaaggtggg gactacccag cagtctatag agcagctcga aaacggtaaa
      241 actaagcgac cacgcttttt accagaactt gcgtcagctc ttggcgtaag tgttgactgg
      301 ctgctcaatg gcacctctga ttcgaatgtt agatttgttg ggcacgttga gcccaaaggg
      361 aaatatccat tgattagcat ggttagagct ggttcgtggt gtgaagcttg tgaaccctac
      421 gatatcaagg acattgatga atggtatgac agtgacgtta acttattagg caatggattc
      481 tggctgaagg ttgaaggtga ttccatgacc tcacctgtag gtcaaagcat ccctgaaggt
      541 catatggtgt tagtagatac tggacgggag ccagtgaatg gaagccttgt tgtagccaaa
      601 ctgactgacg cgaacgaagc aacattcaag aaactggtca tagatggcgg tcagaagtac
      661 ctgaaaggcc tgaatccttc atggcctatg actcctatca acggaaactg caagattatc
      721 ggtgttgtcg tggaagcgag ggtaaaattc gtagctgcaa acgacgaaaa ctacgcttta
      781 gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaagg tggtgaatac
      841 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      901 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      961 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1021 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1081 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1141 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1201 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1261 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1321 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1381 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1441 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     1501 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
     1561 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1621 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1681 tttata
//