LOCUS       BBa_K607002             2154 bp    DNA     linear       23-Nov-2024
DEFINITION  PBad_LasR-LVA
ACCESSION   BBa_K607002
VERSION     BBa_K607002.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1953
                     /label=lasR
     misc_feature    1954..1986
                     /label=LVA
     misc_feature    1716..1716
                     /label=G
     misc_feature    1993..2017
                     /label=Help:Barcodes
     CDS             1237..2017
                     /label=BBa_C0079
     stem_loop       2037..2080
                     /label=stem_loop
     misc_feature    2026..2105
                     /label=BBa_B0010
     terminator      2026..2105
                     /label=BBa_B0010
     stem_loop       2121..2140
                     /label=T7 TE
     misc_feature    2147..2147
                     /label=stop
     polyA_site      2141..2154
                     /label=polya
     misc_feature    2114..2154
                     /label=BBa_B0012
     terminator      2114..2154
                     /label=BBa_B0012
     terminator      2026..2154
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg ccttggttga cggttttctt
     1261 gagctggaac gctcaagtgg aaaattggag tggagcgcca tcctccagaa gatggcgagc
     1321 gaccttggat tctcgaagat cctgttcggc ctgttgccta aggacagcca ggactacgag
     1381 aacgccttca tcgtcggcaa ctacccggcc gcctggcgcg agcattacga ccgggctggc
     1441 tacgcgcggg tcgacccgac ggtcagtcac tgtacccaga gcgtactgcc gattttctgg
     1501 gaaccgtcca tctaccagac gcgaaagcag cacgagttct tcgaggaagc ctcggccgcc
     1561 ggcctggtgt atgggctgac catgccgctg catggtgctc gcggcgaact cggcgcgctg
     1621 agcctcagcg tggaagcgga aaaccgggcc gaggccaacc gtttcataga gtcggtcctg
     1681 ccgaccctgt ggatgctcaa ggactacgca ctgcaaagcg gtgccggact ggccttcgaa
     1741 catccggtca gcaaaccggt ggttctgacc agccgggaga aggaagtgtt gcagtggtgc
     1801 gccatcggca agaccagttg ggagatatcg gttatctgca actgctcgga agccaatgtg
     1861 aacttccata tgggaaatat tcggcggaag ttcggtgtga cctcccgccg cgtagcggcc
     1921 attatggccg ttaatttggg tcttattact ctcgctgcaa acgacgaaaa ctacgcttta
     1981 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
     2041 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2101 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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