LOCUS       BBa_K607014             1245 bp    DNA     linear       14-Nov-2024
DEFINITION  Prm_cI-DAS_antiPlasB with 0.07RBS
ACCESSION   BBa_K607014
VERSION     BBa_K607014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     promoter        208..213
                     /label=-10
     promoter        185..190
                     /label=-35
     promoter        138..219
                     /label=BBa_I12006
     misc_feature    234..237
                     /label=conserved
     RBS             228..241
                     /label=BBa_B0031
     misc_feature    248..958
                     /label=BBa_K607015
     misc_feature    967..999
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    967..999
                     /label=BBa_M0052
     misc_feature    1008..1108
                     /label=misc
     promoter        1008..1108
                     /label=BBa_K607008
     stem_loop       1128..1171
                     /label=stem_loop
     misc_feature    1117..1196
                     /label=BBa_B0010
     terminator      1117..1196
                     /label=BBa_B0010
     stem_loop       1212..1231
                     /label=T7 TE
     misc_feature    1238..1238
                     /label=stop
     polyA_site      1232..1245
                     /label=polya
     misc_feature    1205..1245
                     /label=BBa_B0012
     terminator      1205..1245
                     /label=BBa_B0012
     terminator      1117..1245
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagtca cacaggaaac
      241 ctactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg
      301 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc
      361 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt
      421 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag
      481 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact
      541 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga
      601 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag
      661 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa
      721 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg
      781 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga
      841 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga
      901 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcta
      961 ctagaggctg ctaacgacga aaactacgct gacgcttctt actagaggct ttcgtgtacc
     1021 aaaaagaacc cgcggccaaa cctgccagaa ctggcaggta gccttgattt cgccggaaat
     1081 ctgtatgttt tcgctggaat agctagcata ctagagccag gcatcaaata aaacgaaagg
     1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1201 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//