LOCUS BBa_K607017 1246 bp DNA linear 26-Nov-2024 DEFINITION Prm_cI-DAS_antiPlasB with 0.6RBS ACCESSION BBa_K607017 VERSION BBa_K607017.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 misc_feature 193..206 /label=OR1 434 misc_feature 146..162 /label=OR1 lambda misc_feature 170..186 /label=OR2 lambda promoter 208..213 /label=-10 promoter 185..190 /label=-35 promoter 138..219 /label=BBa_I12006 RBS 228..242 /label=RBS-1\Strong RBS 235..239 /label=RBS misc_feature 228..242 /label=BBa_B0030 RBS 228..242 /label=BBa_B0030 misc_feature 249..959 /label=BBa_K607015 misc_feature 968..1000 /label=AANDENYADAS SsrA degradation tag misc_feature 968..1000 /label=BBa_M0052 misc_feature 1009..1109 /label=misc promoter 1009..1109 /label=BBa_K607008 stem_loop 1129..1172 /label=stem_loop misc_feature 1118..1197 /label=BBa_B0010 terminator 1118..1197 /label=BBa_B0010 stem_loop 1213..1232 /label=T7 TE misc_feature 1239..1239 /label=stop polyA_site 1233..1246 /label=polya misc_feature 1206..1246 /label=BBa_B0012 terminator 1206..1246 /label=BBa_B0012 terminator 1118..1246 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg 181 gtgttagata ttacaaactt tcttgtatag atttaacgtt actagagatt aaagaggaga 241 aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc 301 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg 361 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat 421 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta 481 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac 541 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg 601 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca 661 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca 721 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag 781 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg 841 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg 901 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggct 961 actagaggct gctaacgacg aaaactacgc tgacgcttct tactagaggc tttcgtgtac 1021 caaaaagaac ccgcggccaa acctgccaga actggcaggt agccttgatt tcgccggaaa 1081 tctgtatgtt ttcgctggaa tagctagcat actagagcca ggcatcaaat aaaacgaaag 1141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1201 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //