LOCUS       BBa_K607023             2458 bp    DNA     linear       14-Nov-2024
DEFINITION  PlasB_LasR-LVA_antipBad/araC with 0.07RBS
ACCESSION   BBa_K607023
VERSION     BBa_K607023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     misc_feature    309..312
                     /label=conserved
     RBS             303..316
                     /label=BBa_B0031
     CDS             323..1039
                     /label=lasR
     misc_feature    1040..1072
                     /label=LVA
     misc_feature    802..802
                     /label=G
     misc_feature    1079..1103
                     /label=Help:Barcodes
     CDS             323..1103
                     /label=BBa_C0079
     promoter        1112..2321
                     /label=BBa_K607040
     stem_loop       2341..2384
                     /label=stem_loop
     misc_feature    2330..2409
                     /label=BBa_B0010
     terminator      2330..2409
                     /label=BBa_B0010
     stem_loop       2425..2444
                     /label=T7 TE
     misc_feature    2451..2451
                     /label=stop
     polyA_site      2445..2458
                     /label=polya
     misc_feature    2418..2458
                     /label=BBa_B0012
     terminator      2418..2458
                     /label=BBa_B0012
     terminator      2330..2458
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agtcacacag gaaacctact agatggcctt ggttgacggt tttcttgagc tggaacgctc
      361 aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc
      421 gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt
      481 cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga
      541 cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta
      601 ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg
      661 gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga
      721 agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat
      781 gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa
      841 accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac
      901 cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg
      961 aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa
     1021 tttgggtctt attactctcg ctgcaaacga cgaaaactac gctttagtag cttaataaca
     1081 ctgatagtgc tagtgtagat cactactaga ggctagccca aaaaaacggg tatggagaaa
     1141 cagtagagag ttgcgataaa aagcgtcagg taggatccgc taatcttatg gataaaaatg
     1201 ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt ctgccgtgat
     1261 tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt tacagaatgc
     1321 ttttaataag cggggttacc ggtttggtta gcgagaagag ccagtaaaag acgcagtgac
     1381 ggcaatgtct gatgcaatat ggacaattgg tttcttctct gaatggcggg agtatgaaaa
     1441 gtatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg
     1501 tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc gaccgaccgc
     1561 tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc
     1621 agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca ggagagattc
     1681 atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc
     1741 cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat acggggttct
     1801 ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg
     1861 ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt
     1921 tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac
     1981 gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg
     2041 tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag
     2101 ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga
     2161 gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct
     2221 atttctcgcg ggtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt
     2281 gtgaagaaaa agtgaatgat gtagccgtca agttgtcata atactagagc caggcatcaa
     2341 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2401 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//