LOCUS       BBa_K607024             2457 bp    DNA     linear       25-Nov-2024
DEFINITION  PlasB_LasR-LVA_antipBad/araC with 0.3RBS
ACCESSION   BBa_K607024
VERSION     BBa_K607024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     RBS             303..315
                     /label=RBS-3\Weak
     RBS             309..312
                     /label=RBS
     misc_feature    303..315
                     /label=BBa_B0032
     RBS             303..315
                     /label=BBa_B0032
     CDS             322..1038
                     /label=lasR
     misc_feature    1039..1071
                     /label=LVA
     misc_feature    801..801
                     /label=G
     misc_feature    1078..1102
                     /label=Help:Barcodes
     CDS             322..1102
                     /label=BBa_C0079
     promoter        1111..2320
                     /label=BBa_K607040
     stem_loop       2340..2383
                     /label=stem_loop
     misc_feature    2329..2408
                     /label=BBa_B0010
     terminator      2329..2408
                     /label=BBa_B0010
     stem_loop       2424..2443
                     /label=T7 TE
     misc_feature    2450..2450
                     /label=stop
     polyA_site      2444..2457
                     /label=polya
     misc_feature    2417..2457
                     /label=BBa_B0012
     terminator      2417..2457
                     /label=BBa_B0012
     terminator      2329..2457
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agtcacacag gaaagtacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      361 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      421 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      481 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      541 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      601 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      661 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      721 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      781 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      841 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      901 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      961 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
     1021 ttgggtctta ttactctcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
     1081 tgatagtgct agtgtagatc actactagag gctagcccaa aaaaacgggt atggagaaac
     1141 agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc
     1201 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
     1261 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
     1321 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
     1381 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
     1441 tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg cccatctggt
     1501 ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg accgaccgct
     1561 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca
     1621 gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag gagagattca
     1681 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc
     1741 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cggggttctt
     1801 tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa tcattaacgc
     1861 cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg agcaattgtt
     1921 actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata atcgggtacg
     1981 cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata tcgccagcgt
     2041 cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc agcagttagg
     2101 gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc tgcttttgag
     2161 caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta
     2221 tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc gtgccggttg
     2281 tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa tactagagcc aggcatcaaa
     2341 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2401 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//